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Planta Med 2003; 69(2): 184-186
DOI: 10.1055/s-2003-37713
Letter
© Georg Thieme Verlag Stuttgart · New York

Inhibition of Mutagenicity of Food-Derived Heterocyclic Amines by Sulphoraphene - an Isothiocyanate Isolated from Radish

 Shishu1 , A. K. Singla1 , I. P. Kaur1
  • 1University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh, India.
Further Information

Publication History

Received: July 12, 2002

Accepted: October 20, 2002

Publication Date:
07 March 2003 (online)

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Abstract

The naturally derived isothiocyanate, sulphoraphene [4-isothiocyanato-(1R)-(methylsulphinyl)-1-(E)-butene], isolated from seeds of radish (Raphanus sativus L., Cruciferae) was investigated for its antigenotoxic effects against a battery of cooked food mutagens (heterocyclic amines) in the Ames Salmonella/reversion assay using Salmonella typhimurium TA98 (frame-shift mutation sensitive) and TA100 (base -pair mutation sensitive) bacterial strains in the presence of Aroclor 1254 induced rat liver S9. Results of the present in vitro anti-mutagenicity studies using the base-pair mutation sensitive strain TA100, strongly suggest that sulphoraphene is a potent inhibitor of the S9-mediated mutagenicity of all the tested heterocyclic amines (60 - 75 % inhibition at a dose of 500 nmol/plate).

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 Shishu

University Institute of Pharmaceutical Sciences

Panjab University

Chandigarh-160014

India

Phone: +91-172-534112(O), +91-172-782099(R)

Fax: +91-172-541142

Email: shishugoindi@yahoo.co.in

Email: goindi@satyam.net.in

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