Basic ResearchAltered cellular calcium regulatory systems in a rat model of cirrhotic cardiomyopathy☆,☆☆
Section snippets
Solutions, reagents, and enzymes
DNA synthase, RNA superscript synthase, and polymerase chain reaction buffer were purchased from Gibco BRL, Life Technologies Inc. (Gaithersburg, MD). Agarose was obtained from ICN Biomedicals Inc. (Aurora, OH), anti-RYR antibody was purchased in DSHB (University of Iowa, Iowa City, IA). The standard Tyrode solution contains 140 mmol/L NaCl, 5.4 mmol/L KCl, 1 mmol/L MgCl2, 1 mmol/L Na2HPO4, 5 mmol/L HEPES, and 10 mmol/L glucose; pH was adjusted to 7.4 with 1 mol/L NaOH and continuously gassed
Cardiomyocyte contractility
Isolated cardiomyocyte contractile studies showed that both baseline and isoproterenol-stimulated contractility were significantly decreased in BDL-treated rats compared with sham controls (Figure 1).Baseline values for fractional shortening were 8.0% ± 1.0% in control (n = 11) and 2.8% ± 0.4% in
Discussion
Cardiovascular anomalies associated with hepatic cirrhosis were first described almost half a century ago, with the initial descriptions of hyperdynamic circulation,25 followed in the late 1960s by reports of depressed ventricular contractile function in patients with alcoholic cirrhosis.2 These were believed to be manifestations of mild or latent alcoholic cardiomyopathy. Only in the past 15 years has it become clear that this ventricular dysfunction is associated with cirrhosis per se,
Acknowledgements
Drs. Ward and Liu contributed equally to this work.
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Address requests for reprints to: Samuel S. Lee, M.D., 3330 Hospital Drive NW, Calgary, Alberta T2N 4N1, Canada. e-mail: [email protected]; fax: (403) 270-0995.
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Supported by an operating research grant from the Canadian Institutes of Health Research. Dr. Liu was supported by a Canadian Association for the Study of Liver (CASL)—Amgen Research Fellowship award, and Dr. Lee was supported by an Alberta Heritage Foundation for Medical Research Senior Scholarship award.