Original articleA rodent model of metabolic surgery for study of type 2 diabetes and positron emission tomography scanning of beta cell mass
Section snippets
Rodents
The Institutional Animal Care and Use Committee of the Columbia University College of Physicians and Surgeons approved the study protocol. A total of 53 male GK rats (Taconic Laboratory, Hudson, NY) were used for this study. On arrival at the Animal Care Facility of Columbia University, the rats were 10–12 weeks old and weighed 290–320 g. The rats were housed 2 per cage in a 12-hour dark/light cycle room that was maintained at a temperature of 26°C. The rodents were allowed to acclimate for 7
Surgery
Of the 53 rodents, 10 underwent a sham operation, 10 SG, and 33 DJB. No morbidity or mortality occurred in the sham group. In the SG group, 2 rodents developed an enterocutaneous fistula that responded to conservative management. One developed an abdominal wall abscess that did not respond to incision and drainage and antibiotics; this rodent was euthanized 32 days after surgery. At necropsy, multiple small abscesses in the peritoneal cavity were visualized.
A total of 33 animals underwent DJB.
Discussion
T2DM is a chronic disease for which there is no medical cure. Medical treatment of T2DM centers on dietary modification, increased physical activity, and administration of oral pharmaceutical agents and/or insulin or a combination of these interventions. In a randomized controlled trial comparing dietary, sulfonylurea, metformin, and insulin treatment for T2DM, only 25% of patients were able to achieve a glycosylated hemoglobin <7% at 9 years after treatment initiation [15]. It has long been
Conclusion
The GK rodent model of T2DM is well suited for the study of metabolic surgery. However, GK rodents are extremely fragile and must be handled with great care. A comprehensive perioperative management protocol can help lower the operative mortality. [11C]-DTBZ micro-PET scanning is feasible in rodents with T2DM; however, additional study is needed to determine the accuracy of this functional imaging modality.
Disclosures
W. B. Inabnet, Covidien (research grant, Clinical Advisory Board, fellowship support); Marc Bessler, Covidien (consultant, fellowship support).
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2024, Surgery Open ScienceThe utility of [<sup>11</sup>C] dihydrotetrabenazine positron emission tomography scanning in assessing β-cell performance after sleeve gastrectomy and duodenal-jejunal bypass
2010, SurgeryCitation Excerpt :P ≤ .05 was considered significant. As previously reported, the DJB procedure has an associated mortality of approximately 60%.15 Owing to the high mortality in the DJB cohort, the final recruitment of GK rodents for this study was as follows: control (n = 6), sham (n = 6), SG (n = 10), and DJB (n = 5).
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This study was funded by a research grant from Covidien Healthcare and a research start-up grant from the Department of Surgery, Columbia University College of Physicians and Surgeons, New York, New York