Mutation Research/Genetic Toxicology and Environmental Mutagenesis
Increased DNA damage in patients with complete hydatidiform mole
Section snippets
Background and aims
The gestational trophoblastic diseases (GTD) comprise a group of interrelated diseases arising from placental trophoblastic tissue after normal or abnormal fertilisation. The WHO classification of GTD includes hydatidiform mole (complete and partial), invasive mole, choriocarcinoma, placental site trophoblastic tumour, and miscellaneous and unclassified trophoblastic lesions [1]. Complete hydatidiform mole (CHM) is by far the most commonly observed variant and is a lesion of the placenta
Subjects
Thirty-five women were enrolled in this study. Of these, 12 were healthy women in the first trimester of pregnancy with a single viable foetus (mean gestational age 10.4 weeks as estimated by ultrasonography). Ten healthy non-pregnant women also participated as controls. The remaining 13 subjects had complete hydatidiform mole (CHM) (mean gestational age 10.1 weeks as estimated from the date of the last menstrual period). Diagnosis of CHM was based on histopathological examination of the molar
Comet assay
The demographic characteristics of the subjects are shown in Table 1. There were no differences in mean age, gestational age, gravidity, or parity between the subjects. Between groups, the endogenous levels of DNA damage in the peripheral blood lymphocytes, the plasma TAR, the plasma total peroxide levels and the OSI were found to be significantly different (all p-values < 0.001) according to the ANOVA test. As seen in Table 2, the DNA damage levels were significantly higher in the patients with
Discussion
It is well known that oxidative stress increases during normal pregnancy. The production of reactive oxygen species is enhanced due to the increased metabolic activity during pregnancy, and this increase is even greater when the pregnancy is complicated by conditions such as hypertension or diabetes mellitus [13], [15].
Recently we have shown that in complicated pregnancies (i.e. in preeclampsia or CHM) patients are subjected to higher levels of oxidative stress. This state of oxidative stress
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