AAP PaperChest wall repair with engineered fetal bone grafts: an efficacy analysis in an autologous leporine model
Section snippets
Materials and methods
This study was approved by the Institutional Animal Care and Use Committee of Children's Hospital Boston under protocol number A05-12-104.
Results
There was no mortality or postoperative complication after any of the chest wall reconstructions. At necropsy, complete closure of the defect was evident on gross inspection in all animals with engineered grafts. On the other hand, 3 (60%) of 5 rabbits that received acellular implants displayed evidence of nonunion on gross examination, suggested by a mobile sternum in the area of repair. This difference was not statistically significant on Fisher's Exact test, however, in this small early
Discussion
The current study builds on our prior work demonstrating the feasibility of this therapeutic concept, by providing a first look at the comparative efficacy of aMSC-based bone grafts in experimental chest wall reconstruction [10]. We continued to use the full-thickness sternal repair model here for a couple of reasons. On the one hand, this model does not allow for vascular in-growth into the graft from its dorsal aspect, which faces the (beating) heart, thus mimicking one of the more plausible
Acknowledgments
The authors are indebted to Dr Arthur Nedder, DVM, and Mr Mark Kelly for their exemplary veterinary care.
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Presented at the 61st Annual Meeting of the Section on Surgery, American Academy of Pediatrics, Washington, DC, 2009.