AAP PaperSternal repair with bone grafts engineered from amniotic mesenchymal stem cells☆,☆☆
Section snippets
Materials and methods
This study was approved by the Institutional Animal Care and Use Committee of Children's Hospital Boston under protocol no. A05-12-104.
Results
There were no postoperative complications or mortality after the chest wall reconstructions. Chest roentgenograms performed at 2 months postimplantation showed radiodense material overlying the repaired defect in all rabbits (Fig. 2). Necropsy similarly demonstrated complete closure of the defect in all animals, with the constructs still distinctly visible amid native tissue.
Discussion
The translational implication of the concept explored herein is self-explanatory. Once the diagnosis of a major congenital chest wall anomaly is made through fetal imaging, an autologous osseous graft would be engineered in parallel to gestation, so that the affected neonate could benefit from its availability immediately after birth. Given that a diagnostic amniocentesis is routinely indicated in the setting of any major congenital structural anomaly, combined with the anticipated autologous
Acknowledgments
The authors thank Dr Arthur Nedder and Mr Mark Kelly for their exemplary veterinary care.
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Cited by (0)
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SAS was supported by the Joshua Ryan Rappaport Fellowship of the Department of Surgery, Children's Hospital Boston (Mass).
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Presented at the 60th Annual Meeting of the Section on Surgery, American Academy of Pediatrics, Boston, Mass, October 10-12, 2008.