Original articleRelationship among cardiac index, inspiration/expiration ratio, and perfluorocarbon dose during partial liquid ventilation in an oleic acid model of acute lung injury in sheep☆,
Section snippets
Animal preparation
After review and approval by the Regierungspräsidium Karlsruhe, Germany, the study was carried out as a prospective, randomized controlled trial in the animal laboratories of the Klinikum Mannheim University Medical Center. University of Heidelberg guidelines for the use and care of animals were followed throughout. After intramuscular premedication with 2 mL Xylazin (Rompun 2%, Bayer Leverkusen, Germany), anesthesia was started by intravenous injection of 3 mg/kg ketamine (Ketanest,
Fick and QTD techniques comparison
In a total of 98 simultaneous measurements, mean QTD was 1.40 ± 0.07 L/min · m2 and mean QFick was 1.51 ± 0.06 L/min · m2. Correlation between techniques was r = 0.82 and Bland-Altman analysis revealed variation reliability between techniques with a mean difference of 0.21 ± 0.15 L/min · m2 (Fig. 1). However, the paired samples t test revealed significant differences between techniques at P = .001. Progressive doses of perflubron stepwise from 10 to 40 mL/kg had no significant influence on the
Discussion
At induction of acute lung injury, PEEP was set to and maintained at 12 cm H2O based on the results of previous studies in oleic-acid injured sheep during PLV [25]. It was shown that PEEP of more than 12 cm H2O is sufficient to maintain airway pressures above the lower inflection point (LIP) of the pressure-volume curve [13]. All other ventilatory parameters besides I/E ratio were kept constant among all investigational groups.
Improvements in pulmonary parameters during perfluorocarbon PLV in
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This study was supported in part by departmental funds of the Klinikum Mannheim University Medical Center. Perflubron was generously provided by the Alliance Pharmaceutical Corp, San Diego, Calif.
This work is attributed to and was performed at the Animal Research Laboratories, Universitätsklinikum Mannheim, Fakultät für klinische Medizin der Universität Heidelberg, Mannheim, Germany. This study was presented in part at the 1999 meeting of the European Society of Intensive Care Medicine [Int Care Med. 1999;25(suppl 1):199].