Journal of Plastic, Reconstructive & Aesthetic Surgery
Improvement of skin-graft survival after autologous transplantation of adipose-derived stem cells in rats
Section snippets
Materials and methods
Twenty male Sprague–Dawley rats, weighing between 350 and 450 g, were used in this study. The National Research Council’s guidelines for the care and use of laboratory animals were followed. The experiments were performed under general anaesthesia using ketamine hydrochloride (100 mg kg−1 intramuscularly) and xylazine (5 mg kg−1 intramuscularly). Antibiotic prophylaxis with cefamandole nafate (20 mg kg−1 intramuscularly) and analgesia with carprofen (0.08 ml kg−1 subcutaneously) were used prior
ASCs’ immunophenotype and differentiation
Flow cytometry analysis showed that cultured cells of passage 1 were positive for stromal markers CD29 (integrin unit) and CD44 (receptor for hyalouronic acid) and negative for haematopoietic markers CD45 (pan-leucocyte marker), CD31 (differentiated endothelial marker) and CD34 (marks primitive haematopoietic progenitors) with 10–20% CD34(+). ASCs differentiated into adipocytes, chondrocytes and osteocytes, as assessed by the specific staining (Figure 1).
Skin-graft survival assessment
At 7 days postoperatively, the regions
Discussion
Our study evaluated the effect of autologous transplantation of ASCs in survival and wound healing promotion of full-thickness skin grafts in rats, with a statistically significant increase in skin-graft survival area in ASC-treated animals compared with controls. Although mean necrosis in our control group (32.62%) was relatively high compared with humans, this is in accordance with skin-graft necrosis in control groups in rat models in other studies.5, 6
Full-thickness skin-graft survival in
Ethical approval of the study
This study had the ethical approval of the Institutional Ethical Committee of the University of Athens and the ethical approval of the Hellenic Veterinary Medicine Committee.
Conflict of interest statement
None of the authors has a financial interest in any of the products, devices or drugs mentioned in this article.
Acknowledgements
This study was partially supported by the ‘Kapodistrias’ research programme of Athens University and by the ‘Bodossakis Foundation’ grant.
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