Scientific (Exp)/researchIntracellular delivery of adenosine triphosphate enhanced healing process in full-thickness skin wounds in diabetic rabbits
Section snippets
Preparation of ATP-vesicles
The ATP-vesicles were made by Avanti Polar Lipids, Inc. (Alabaster, AL). The freeze-dried ATP-vesicles were stored in a freezer at −20°C. They were mixed with a nonionic vanishing cream (Dermovan, San Antonio, TX) just before use. After reconstitution, the composition was 100 mg/mL of soy PC/DOTAP (Avanti Polar Lipids, Alabaster, AL) (50:1), trehalose/soy PC (2:1), 10 mmol/L of KH2PO4, and 10 mmol/L of Mg–ATP. The diameters of the lipid vesicles ranged from 120 nm to 160 nm, which were measured
Results
There was no death, bleeding, or incisional infection in these animals. Among the 8 rabbits (64 wounds) that were monitored until all wounds were healed, 2 wounds (1 treated by ATP-vesicles and 1 treated by saline) on the ischemic ear in 1 rabbit were infected. These 2 wounds healed extremely slowly, and were excluded from wound healing time analysis. The remaining 62 wounds were used for healing comparison.
Comments
The important findings of this experiment include the following: (1) wound experiment can be performed successfully in rabbits with the combination of diabetes, ischemia, and nerve damage; (2) the delivery of ATP into the cell can accelerate the healing process of ischemic and non-ischemic skin wounds in diabetic rabbits; (3) the extremely rapid granulation tissue growth in the diabetic animals in this study is something never seen or reported in humans or any other land animals in the past;
Acknowledgments
This publication was made possible in part by Grant No. DK74566 from the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), and AR52984 from the National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS). We thank Sheron Lear in the Special Procedures Laboratory for her sample preparations for histological study.
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Cited by (0)
Sufan Chien is the partner of Novera, LLC in Louisville, which plans to further develop and commercialize the ATP-vesicle for wound treatment.