Alimentary TractPresence of adherent Escherichia coli strains in ileal mucosa of patients with Crohn's disease☆,☆☆
Section snippets
Patients
Sixty-three mucosal specimens were studied, 50 from patients with CD and 13 from controls.
Quantification of E. coli strains in ileal mucosa
E. coli strains were isolated from 13 (65%) of the 20 biopsy specimens of chronic ileal lesions in patients with CD. Similar percentages were obtained from healthy ileal mucosa of patients with CD (64%) and controls (69%). This percentage reached 100% in the early ileal lesions of the 19 patients who had endoscopic recurrence. To estimate the colonization of the ileal mucosa by E. coli, we determined the percentage of E. coli in the total bacteria present in the specimens. E. coli was
Discussion
Various studies have addressed the hypothesis that virulent or adherent E. coli contributes to the pathogenesis of inflammatory bowel disease in an unexplained manner.19, 20, 21, 22 Conflicting results were obtained, possibly because the strains analyzed were isolated from feces or rectal biopsy specimens. In the present study, to our knowledge the first one of its type, we assessed the prominence and virulence of E. coli strains associated with the ileal mucosa, the most important site of CD.
Acknowledgements
The authors thank Michael Donnenberg for providing plasmid pCVD434, Myron M. Levine for the EAggEC strain 17-2 and plasmid pCVD432, Inga Benz for plasmid pIB6, James Nataro for plasmid pJPN16, and Steve Moseley for plasmids pEWD299 and pSLM852 and the E. coli strain C1845. They also thank Chantal Le Bouguenec for providing the recombinant plasmids pILL22, pILL1019, pILL61; Chantal Rich and Agnès Couteau for excellent technical assistance; Christophe De Champs for statistical analysis of the
References (53)
- et al.
Interleukin-10-deficient mice develop chronic enterocolitis
Cell
(1993) - et al.
Ulcerative colitis-like disease in mice with a disrupted interleu-kine-2 gene
Cell
(1993) - et al.
Effect of faecal stream diversion on recurrence of Crohn's disease in the neoterminal ileum
Lancet
(1991) - et al.
Immunocytochemical evidence of Listeria, Escherichia coli and Streptococcus antigens in Crohn's disease
Gastroenterology
(1995) - et al.
Predictability of the postoperative course of Crohn's disease
Gastroenterology
(1990) - et al.
Detection by molecular hybridization of pap, afa, and sfa adherence systems in Escherichia coli strains associated with urinary and enteral infections
Ann Inst Pasteur Microbiol Paris
(1988) - et al.
Colonic glycoproteins in monozygotic twins with inflammatory bowel disease
Gastroenterology
(1991) Unifying hypothesis for inflammatory bowel disease and associated colon cancer: sticking the pieces together with sugar
Lancet
(1996)- et al.
Escherichia coli O157:H7 infection mimicking Crohn's disease
Gastroenterology
(1997) Enteric microflora in IBD: pathogens or commensals?
Inflam Bowel Dis
(1997)
Microbial factors in the pathogenesis of Crohn's disease, ulcerative colitis and experimental intestinal inflammation
Normal bacteria stimulate colonic, gastric, and systemic inflammation in HLA-B27/β2-m transgenic rats (abstr)
Gastroenterology
The germfree state prevents development of gut and joint inflammatory disease in HLA-B27 transgenic rats
J Exp Med
Absence of gastritis and colitis in germ-free IL-10 knockout mice (abstr)
Gastroenterology
IL-2 deficient mice raised under germfree conditions develop delayed mild focal intestinal inflammation and progressive loss of B cells (abstr)
Gastroenterology
Bacterial overgrowth in the neoterminal ileum after ileocolonic resection for Crohn's disease (abstr)
Gastroenterology
Escherichia coli antibodies in patients with inflammatory bowel disease
Gut
Incidence of pathogenic bacteria from mesenteric lymph nodes and ileal serosa during Crohn's disease surgery
Br J Surg
Translocation bactérienne dans la maladie de Crohn
Gastroenterol Clin Biol
An immunocytochemical search for infectious agents in Crohn's disease
Mod Pathol
Escherichia coli that cause diarrhea: enterotoxigenic, enteropathogenic, enteroinvasive, enterohemorrhagic and enteroadherent
J Infect Dis
Escherichia coli strains involved in diarrhea in France: high prevalence and heterogeneity of diffusely adhering strains
J Clin Microbiol
Patterns of adherence of diarrheagenic Escherichia coli to HEp-2 cells
Pediatr Infect Dis J
Adhesive Escherichia coli in inflammatory bowel disease and infective diarrhoea
BMJ
Virulence properties of Escherichia coli strains isolated from patients with inflammatory bowel disease
Gut
Expression of binding of plasminogen, thrombospondin, vitronectin, and fibrinogen, and adhesive properties by Escherichia coli strains isolated from patients with colonic diseases
Gut
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Address requests for reprints to: Arlette Darfeuille-Michaud, Ph.D., Laboratoire de Bactériologie, Faculté de Pharmacie, 28 Place Henri Dunant, 63001 Clermont-Ferrand Cedex, France. Fax: (33) 4-7327-7494; e-mail: [email protected].
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Supported by grants from the Association F. Aupetit, the Fonds de Recherche de la Société Nationale Française de Gastro-Entérologie, the Centre Hospitalier Régional Universitaire de Lille (contract 96/38/9713), the Ministère de la Recherche et de la Technologie (EA522), and INSERM CRI 4U004B.