Neuron
Volume 9, Issue 2, August 1992, Pages 295-305
Journal home page for Neuron

Article
Regulation of ciliary neurotrophic factor expression in myelin-related Schwann cells in vivo

https://doi.org/10.1016/0896-6273(92)90168-DGet rights and content

Abstract

Adult rat sciatic nerve is known to express high levels of ciliary neurotrophic factor (CNTF) mRNA and protein. Here we examine the cellular localization of CNTF protein and mRNA in peripheral nerve and the regulation of CNTF expression by peripheral axons. In intact nerve, CNTF immunoreactivity is found predominantly in the cytoplasm of myelin-related Schwann cells. After axotomy, CNTF immunoreactivity and mRNA levels fall dramatically and do not recover unless axons regenerate. This behavior is similar to the pattern of myelin gene expression in these nerves. We conclude that the expression of CNTF in Schwann cells depends on axon-Schwann cell interactions.

References (58)

  • P.M. Richardson et al.

    Nerve growth activities in rat peripheral nerve

    Brain Res.

    (1982)
  • H.J. Weinberg et al.

    Studies on the control of myelinogenesis. II. Evidence for neuronal regulation of myelin production

    Brain Res.

    (1976)
  • L.R. Williams et al.

    High ciliary neuronotrophic specific activity in rat peripheral nerve

    Int. J. Dev. Neurosci.

    (1984)
  • H.J. Willison et al.

    The expression of myelin-associated glycoprotein during remyelination in cat sciatic nerve

    Brain Res.

    (1988)
  • J. Ygge

    Neuronal loss in dorsal root ganglia after proximal compared to distal resection. A quantitative study in the rat

    Brain Res.

    (1989)
  • R. Adler et al.

    Cholinergic neuronotrophic factors. Intraocular distribution of soluble trophic activity for ciliary neurons

    Science

    (1979)
  • A.J. Aguayo et al.

    Potential of Schwann cells from unmyelinated nerves to produce myelin. A quantitative ultrastructural and radioautographic study

    J. Neurocytol.

    (1976)
  • Y. Arakawa et al.

    Survival effects of ciliary neurotrophic factor (CNTF) on chick embryonic motoneurons in culture. Comparison with other neurotrophic factors and cytokines

    J. Neurosci.

    (1990)
  • C. Bandtlow et al.

    Cellular localization of nerve growth factor synthesis by in situ hybridization

    EMBO J.

    (1987)
  • G. Barbin et al.

    Purification of the chick eye ciliary neurotrophic factor

    Neurochemistry

    (1984)
  • E.P. Bosch et al.

    Axonal signals regulate expression of glia maturation factor β in Schwann cells, an immunohistochemical study of injured sciatic nerves and cultured Schwann cells

    J. Neurosci.

    (1989)
  • J. Carlson et al.

    Axonal atrophy from permanent axotomy in the adult cat

    J. Neuropathol. Exp. Neurol.

    (1979)
  • J.M. Chirgwin et al.

    Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease

    Biochemistry

    (1979)
  • S. Davis et al.

    The receptor for ciliary neurotrophic factor

    Science

    (1991)
  • P. Fort et al.

    Various adult rat tissues express only one major mRNA species from the glyceraldehyde3-phosphate dehydrogenase multigene family

    Nucl. Acids Res.

    (1985)
  • B. Gold et al.

    Regulation of axonal caliber, neurofilament content and nuclear localization in mature sensory neurons by nerve growth factor

    J. Neurosci.

    (1991)
  • O. Haub et al.

    Expression of the murine fibroblast growth factor 5 gene in the adult central nervous system

  • R. Heumann et al.

    Differential regulation of mRNA encoding nerve growth factor and its receptor in rat sciatic nerve during development, degeneration, and regeneration. Role of macrophages

  • R. Heumann et al.

    Changes of nerve growth factor synthesis in nonneuronal cells in response to sciatic nerve transection

    J. Cell Biol.

    (1988)
  • Cited by (318)

    View all citing articles on Scopus
    View full text