Elsevier

Methods in Enzymology

Volume 186, 1990, Pages 421-431
Methods in Enzymology

[43] Malondialdehyde determination as index of lipid Peroxidation

https://doi.org/10.1016/0076-6879(90)86135-IGet rights and content

Publisher Summary

This chapter describes the malondialdehyde (MDA) as index of lipid peroxidation. The determination of malondialdehyde (MDA) has attracted widespread interest, because it appears to offer a facile means of assessing lipid peroxidation in biological materials. Malondialdehyde occurs in biological materials in free state and in various covalently bound forms. Urine also contains small amounts of MDA adducts with guanine, the phospholipid bases serine and ethanolamine, and other unidentified reactants. Free MDA is a minor and variable excretory product. It is apparent from the occurrence of these derivatives in urine that MDA forms adducts with proteins, nucleic acids, and other substances in vivo, and this compromises the assessment of lipid peroxidation in the tissues based on the determination of free MDA. The pH required for maximum yield of MDA varies among biological materials depending on the nature of the derivatives present. MDA may be generated during hydrolysis by the oxidation of polyunsaturated fatty acids (PUFA) in the sample and by the degradation of preexisting oxidation products. Pigments present in the sample, or generated during hydrolysis, also can interfere in the colorimetric assessment of MDA. These problems, and possibilities for their resolution, are discussed in the chapter.

References (24)

  • L.G. McGirr et al.

    J. Biol. Chem.

    (1985)
  • C. Largilliére et al.

    Anal. Biochem.

    (1988)
  • L.J. Marnett et al.

    Anal. Biochem.

    (1979)
  • A.W. Bull et al.

    Anal. Biochem.

    (1985)
  • B. Halliwell et al.

    Arch. Biochem. Biophys.

    (1986)
  • E.N. Frankel et al.

    Biochim. Biophys. Acta

    (1983)
  • R.P. Bird et al.

    Anal. Biochem.

    (1983)
  • M. Beljean-Leymarie et al.

    Anal. Biochem.

    (1988)
  • K. Kikugawa et al.

    Anal. Biochem.

    (1988)
  • L.A. Pichd et al.

    Carcinogenesis

    (1988)
  • H.H. Draper et al.

    Lipids

    (1988)
  • M.A. Warso et al.

    Clin. Physiol. Biochem.

    (1984)
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