Elsevier

Analytical Biochemistry

Volume 16, Issue 2, August 1966, Pages 359-364
Analytical Biochemistry

Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems

https://doi.org/10.1016/0003-2697(66)90167-9Get rights and content

Abstract

A new thiobarbituric acid method for the estimation of the malonyl dialdehyde produced as a result of lipid peroxidation is described in which the trimethine colored substance is estimated in alkaline solution.

Alkaline pyridine-butanol mixture dissolves both proteins and lipids that are precipitated or opalescent during the reaction, yielding a more accurate MDA estimation as well as simplifying the procedure.

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Cited by (0)

This investigation was supported in part by NIH grant No. 10284.

2

Present address: Biochemistry Section, Oklahoma Medical Research Foundation, 825 Northeast Thirteenth Street, Oklahoma City,Oklahoma 73104.

3

Present address: Department of Biochemistry, University of Oklahoma School of Medicine, 800 Northeast Thirteenth Street, Oklahoma City, Oklahoma, 73104.

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