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Identification of crucial genes related to postmenopausal osteoporosis using gene expression profiling

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Abstract

Background

Postmenopausal osteoporosis is a common bone disease and characterized by low bone mineral density.

Aim

This study aimed to reveal key genes associated with postmenopausal osteoporosis (PMO), and provide a theoretical basis for subsequent experiments.

Methods

The dataset GSE7429 was obtained from Gene Expression Omnibus. A total of 20 B cell samples (ten ones, respectively from postmenopausal women with low or high bone mineral density (BMD) were included in this dataset. Following screening of differentially expressed genes (DEGs), coexpression analysis of all genes was performed, and key genes in the coexpression network were screened using the random walk algorithm. Afterwards, functional and pathway analyses were conducted. Additionally, protein–protein interactions (PPIs) between DEGs and key genes were analyzed.

Results

A set of 308 DEGs (170 up-regulated ones and 138 down-regulated ones) between low BMD and high BMD samples were identified, and 101 key genes in the coexpression network were screened out. In the coexpression network, some genes had a higher score and degree, such as CSTA. The key genes in the coexpression network were mainly enriched in GO terms of the defense response (e.g., SERPINA1 and CST3), immune response (e.g., IL32 and CLEC7A); while, the DEGs were mainly enriched in structural constituent of cytoskeleton (e.g., CYLC2 and TUBA1B) and membrane-enclosed lumen (e.g., CCNE1 and INTS5). In the PPI network, CCNE1 interacted with REL; and TUBA1B interacted with ESR1.

Conclusions

A series of interactions, such as CSTA/TYROBP, CCNE1/REL and TUBA1B/ESR1 might play pivotal roles in the occurrence and development of PMO.

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Correspondence to Feng Yin or Junfeng Cai.

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On behalf of all authors, the corresponding author states that there is no conflict of interest.

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For this type of study, approvement of ethics committee is not required.

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M. Ma, X. Chen and L. Lu should be regarded as co-first authors.

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Ma, M., Chen, X., Lu, L. et al. Identification of crucial genes related to postmenopausal osteoporosis using gene expression profiling. Aging Clin Exp Res 28, 1067–1074 (2016). https://doi.org/10.1007/s40520-015-0509-y

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  • DOI: https://doi.org/10.1007/s40520-015-0509-y

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