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Hsa-miR-132 Regulates Apoptosis in Non-Small Cell Lung Cancer Independent of Acetylcholinesterase

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Abstract

MiR-132 is enriched in the central nerve system and is thought to be involved in neuronal development, maturation and function, and to be associated with several neurological disorders including Alzheimer’s disease. In addition to its documented neuronal functions, an emerging role for miR-132 in tumorigenesis has been suggested. Recently, hsa-miR-132 was shown to be modulated in different tumor types. However, its role in non-small cell lung cancer (NSCLC) remains unclear. Here, we show that hsa-miR-132 can initiate apoptosis in NSCLC cells to dramatically attenuate tumor formation in nude mice independent of its effect on the proliferation/apoptosis-associated gene, acetylcholinesterase (AChE). Interestingly, hsa-miR-132 has no pro-apoptotic effect in normal pulmonary trachea epithelium. Taken together, these results suggest that hsa-miR-132 represses NSCLC growth by inducing apoptosis independent of AChE.

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Acknowledgments

We thank Doctor Hongpin Zheng (Shanghai Institute of Biochemistry and Cell Biology, Shanghai, China) for proving dual luciferase reporter plasmid.

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Authors and Affiliations

  1. State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China

    Bo Zhang, Lu Lu, Xuejin Zhang, Weiyuan Ye, Jun Wu, Qiliang Xi & Xuejun Zhang

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  1. Bo Zhang
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Correspondence to Xuejun Zhang.

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Bo Zhang and Lu Lu are authors who contributed equally to this work.

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Supplementary Figure 1

Hsa-miR-132 triggers apoptosis in H520 cells. (a) Quantitative analysis of the percentage of sub-G1N520 cells following transfection with hsa-miR-132 mimics at the indicated time points post-transfection. (b) TUNEL assays of H520 at indicated time points post-transfection. Scale bar indicates 25 μm in length. (c) Quantitative analysis of TUNEL positive cells in (b). (d) Western blot of cleaved-PARP in H520 cells at indicated time points post-transfection. The results are presented as the mean ± SD. **p < 0.01 (JPEG 71 kb).

High resolution (TIFF 36015 kb).

Supplementary Figure 2

Hsa-miR-132 triggers apoptosis in H460 cells. Western blot of (a) cleaved-PARP and (b) cleaved-caspase3/9 in H460 cells at the indicated time points following transfection. (JPEG 13 kb).

High resolution (TIFF 8236 kb).

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Zhang, B., Lu, L., Zhang, X. et al. Hsa-miR-132 Regulates Apoptosis in Non-Small Cell Lung Cancer Independent of Acetylcholinesterase. J Mol Neurosci 53, 335–344 (2014). https://doi.org/10.1007/s12031-013-0136-z

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