Abstract
Bloodstain pattern analysis (BPA) refers to the collection, categorization and interpretation of the shape and distribution of bloodstains connected with a crime. These kinds of stains occur in a considerable proportion of homicide cases. They offer extensive information and are an important part of a functional, medically and scientifically based reconstruction of a crime. The following groups of patterns can essentially be distinguished: dripped and splashed blood, projected blood, impact patterns, cast-off stains, expirated and transferred bloodstains. A highly qualified analysis can help to estimate facts concerning the location, quality and intensity of an external force. A sequence of events may be recognized, and detailed questions connected with the reconstruction of the crime might be answered. In some cases, BPA helps to distinguish between accident, homicide and suicide or to identify bloodstains originating from a perpetrator. BPA is based on systematic training, a visit to the crime scene or alternatively good photographic documentation, and an understanding and knowledge of autopsy findings or statements made by the perpetrator and/or victim. A BPA working group has been established within the German Society of Legal Medicine aiming to put the knowledge and practical applications of this subdiscipline of forensic science on a wider basis.
References
Brinkmann B. Expertisen an biologischen Spuren–Bestandsaufnahme, zukünftige. Trends Z Rechtsmed. 1988;100:39–54.
Karger B, Nusse R, Bajanowski T. Backspatter on the firearm and hand in experimental close-range gunshots to the head. Am J Forensic Med Pathol. 2002;23(3):211–3.
Karger B, Rand SP, Brinkmann B. Experimental bloodstains on fabric from contact and from droplets. Int J Legal Med. 1998;111(1):17–21.
MacDonell HL. Bloodstain patterns. New York: Laboratory of Forensic Science; 1993.
James SH, Kish PE. Sutton TP principles of bloodstain pattern analysis—theory and practice. Boca Raton: CRC Press; 2005.
Bevel T, Gardner RM. Bloodstain pattern analysis. 2nd ed. With an introduction to crime scene reconstruction. Boca Raton: CRC Press; 2002.
MacDonell HL. Bloodstain pattern interpretation. New York: Laboratory of Forensic Science Publishers; 1982.
Gardener RM. Directionality in swipe patterns. J Forensic Ident. 2002;52(2):579.
Benecke M, Barksdale L. Distinction of bloodstain patterns from fly artefacts. Forensic Sci Int. 2003;137(2–3):152–9.
Carter AL. The directional analysis of bloodstain patterns—theory and experimental validation. Can Soc Forensic Sci J. 2001;34(4):173–89.
Lytle LT, Hedgecock DG. Chemiluminescence in the visualization of forensic bloodstains. J Forensic Sci. 1978;23:550–5.
Grodsky M, Wright K, Kirk PL. Simplified preliminary blood testing. An improved technique and a comparative study of methods. J Crimin Law Criminol Police Sci. 1951;42:95–104.
Weber K. Die Anwendung der Chemilumineszenz des Luminols. Z Gerichtl Medizin. 1995;57:410.
Klein A, Feudel E, Türk E, et al. Lumineszenz nach Luminolanwendung. Richtig- oder falsch- positiv? Z Rechtsmedizin. 2007;17:146–52.
Barni F, Lewis SW, Berti A, Miskelly GM, Lago G. Forensic application of the luminol reaction as a presumptive test for latent blood detection. Talanta. 2007;72:896–913.
Laux DL. Effects on luminol on the subsequent analysis of bloodstains. J Forensic Sci. 1991;36:1512.
Laux DL. The detection of blood using luminol. In: James S, Kish PE, Sutton TP, editors. Principles of bloodstain pattern analysis: theory and practice. Boca Raton: CRC Press; 2005. p. 369–89.
Creamer JI, Quickenden TI, Crichton LB, Robertson P, Ruhayel RA. Attempted cleaning of bloodstains and its effect on the forensic luminol test. Luminescence. 2005;20:411–3.
Wolson TL. Documentation of bloodstain pattern evidence. J Forensic Ident. 1995;45(4):396–408.
Illes MR, Carter AL, Laturnus PL, Yamashita AB. Use of the BackTrack™ Computer Program for bloostain pattern analysis of stains from downward-moving drops. Can Soc Forensic Sci. 2005;38(4):213–8.
Carter AL, Forsythe-Erman J, Hawkes V, et al. Validation of the BackTrack suite of programs for bloodstain pattern analysis. J Forensic Ident. 2006;56(2):242–54.
Author information
Authors and Affiliations
Corresponding author
Appendices
Appendix: CME Questionnaire
-
1.
What shape does a free falling droplet have?
□ Ellipsoid.
□ Sphere.
□ Tear-drop.
□ Long.
□ Triangular.
-
2.
Which statement about the behavior of blood is correct?
□ Small droplets fly over a longer distance than bigger ones.
□ Blood droplets always have the same volume, independently from the shape and surface of the subject they originate from.
□ Falling or accelerated blood oscillates a little bit.
□ The viscosity of blood is slightly higher than that of glycerine with the same temperature.
□ Free-falling blood droplets separate into smaller droplets when falling from a height of 3 m or more.
-
3.
When a flying drop of blood hits a surface four phases of impact can be differentiated. Which of the following is wrong?
□ Diffusion.
□ Contact.
□ Shifting.
□ Dispersion.
□ Retraction.
-
4.
Which of the following blood stain patterns are not projected patterns?
□ Arterial pattern.
□ Cast-off pattern.
□ Exhaled or expirated patterns.
□ Inhaled pattern.
□ Passive drop.
-
5.
What can be determined from a bloodstain pattern?
□ The surface structure of the point of origin.
□ Angle of impact.
□ Age of the blood.
□ Human or animal blood.
□ Blood of a living or a dead person.
-
6.
What typical form can be seen in a cast-off pattern?
□ Y-shape.
□ Double punctuation.
□ Question mark.
□ Exclamation mark.
□ Slash.
-
7.
Which statements considering primary and secondary wipe patterns are true?
□ A primary wipe pattern is created when a bloody object is moved tangentially on a surface leaving a trace (so called swipe pattern).
□ Secondary wipe patterns occur when an uncontaminated, clean surface or object is tangentially drawn over a pre-existing bloodstain (so called wipe pattern).
□ Wipe patterns can be helpful to determine a chronological order of various incidents.
□ Secondary wipe patterns are almost always the result of arterial bleeding.
□ Primary wipe pattern can only be created by arterial bleeding.
-
8.
Which sentence is incorrect?
□ If the object in question has been removed, it might still be possible to reconstruct its shape by examining the outskirts of the void.
□ Blood will usually begin to dry from the outer parts inwards, toward the center of the stain.
□ Projected blood stain patterns are produced when blood is spread on a target under pressure as opposed to an impact.
□ Arterial patterns dry faster than venous ones.
□ A blood clot describes the final product of blood coagulation.
-
9.
Which of the following statements are incorrect?
□ Luminol is used to identify minor, unnoticed or hidden bloodstains.
□ Luminol gives a positive reaction to some vegetables and metals.
□ After having used Luminol a DNA-analysis is not possible.
□ In order to get a perfect result when using Luminol it is important to light up the room.
□ The mixed Luminol solution is directly sprayed onto suspicious areas.
-
10.
Which statement considering documentation is wrong?
□ Photos should always include a standardized scale and a fixed object in reference to the blood stains documented.
□ In order to maintain an objective perspective, an upright position of the camera in reference to the surface being photographed is essential.
□ If a crime scene is not accessible, a blood stain pattern analysis might be possible by photographs alone.
□ Overview shots are not important, since the details of a pattern matter, when calculating the angle of impact.
□ If necessary, physical evidence on hard, immovable objects can be transferred from the crime scene and looked at separately.
CME Questionnaire answers
-
1.
Sphere
-
2.
Falling or accelerated blood oscillates a little bit
-
3.
Diffusion
-
4.
Inhaled pattern
AND
Passive drop
-
5.
Angle of impact
AND
Blood of a living or a dead person
-
6.
Exclamation mark
-
7.
A primary wipe pattern is created when a bloody object is moved tangentially on a surface leaving a trace (so called swipe pattern).
AND
Secondary wipe patterns occur when an uncontaminated, clean surface or object is tangentially drawn over a pre-existing bloodstain (so called wipe pattern).
AND
Wipe patterns can be helpful to determine a chronological order of various incidents
-
8.
Arterial patterns dry faster than venous ones
-
9.
After having used Luminol a DNA-analysis is not possible
AND
In order to get a perfect result when using Luminol it is important to light up the room
-
10.
Overview shots are not important, since the details of a pattern matter, when calculating the angle of impact.
Rights and permissions
About this article
Cite this article
Peschel, O., Kunz, S.N., Rothschild, M.A. et al. Blood stain pattern analysis. Forensic Sci Med Pathol 7, 257–270 (2011). https://doi.org/10.1007/s12024-010-9198-1
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12024-010-9198-1