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Increased Oxidant Stress and Decreased Antioxidant Status in Erythrocytes of Rats Fed with Zinc-deficient Diet

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Abstract

The aim of this study was to evaluate the lipid peroxidation, nitric oxide (NO), and free radical scavenging enzyme activities in erythrocytes of zinc (Zn)-deficient rats and to investigate the relationship among these parameters in either group. Sixteen male rats with a weight of 40–50 g were used for the experiment. The rats were divided into control (n = 8) and Zn-deficient groups. At the end of the experiment, the animals were anesthetized with ketamine–HCl (Ketalar, 20 mg/kg−1, i.p.), and the blood was collected by cardiac puncture after thoracotomy. Blood samples were collected in vacutainer tubes without and with K3-EDTA as anticoagulant. Erythrocyte catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GRD), glutathione-S-transferase (GST), superoxide dismutase (SOD) activities, total (enzymatic plus nonenzymatic) superoxide scavenger activity (TSSA), nonenzymatic superoxide scavenger activity (NSSA), antioxidant potential (AOP), and serum zinc (Zn) values in the Zn-deficient group were significantly lower than those of the control group, whereas NO and malondialdehyde (MDA) levels were significantly higher than those of the control group. The results show that Zn deficiency causes a decrease in antioxidant defense system and an increase in oxidative stress in erythrocyte of rats.

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Authors and Affiliations

  1. Department of Biochemistry, Nenehatun Obstetric and Gynecology Hospital, Erzurum, Turkey

    Seyithan Taysi & Adnan Yilmaz

  2. Department of Surgery, Van State Hospital, Van, Turkey

    Oztekin Cikman

  3. Department of Internal Medicine, Veterinary Faculty, 100 Yıl University, Van, Turkey

    Abdullah Kaya

  4. Department of Biochemistry, School of Medicine, Ataturk University, Erzurum, Turkey

    Berna Demircan & Mehmet Akyuz

  5. Department of Physiology, School of Medicine, Ataturk University, Erzurum, Turkey

    Kenan Gumustekin

  6. Department of Surgery, Elazig Research and Educational Hospital, Elazig, Turkey

    Abdullah Boyuk

  7. Department of Internal Medicine, School of Medicine, Ataturk University, Erzurum, Turkey

    Mustafa Keles & Mehmet Turkeli

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  1. Seyithan Taysi
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  2. Oztekin Cikman
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  3. Abdullah Kaya
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  4. Berna Demircan
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  5. Kenan Gumustekin
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  6. Adnan Yilmaz
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  7. Abdullah Boyuk
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  8. Mustafa Keles
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  9. Mehmet Akyuz
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  10. Mehmet Turkeli
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Correspondence to Seyithan Taysi.

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Taysi, S., Cikman, O., Kaya, A. et al. Increased Oxidant Stress and Decreased Antioxidant Status in Erythrocytes of Rats Fed with Zinc-deficient Diet. Biol Trace Elem Res 123, 161–167 (2008). https://doi.org/10.1007/s12011-008-8095-x

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  • DOI: https://doi.org/10.1007/s12011-008-8095-x

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