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The Role of HLA DQ2 and DQ8 in Dissecting Celiac-Like Disease in Common Variable Immunodeficiency

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Abstract

Objectives

Gastrointestinal manifestations are frequent in patients with common variable immunodeficiency (CVID), and some of the patients present with celiac-like features. Diagnosing celiac disease (CD) in CVID however is challenging, as autoantibody detection and histopathology of the small intestine cannot reliably discriminate between classic CD and a celiac-like disease in these individuals. For the development of classic gluten-sensitive CD a certain HLA haplotype involving the loci DQA1* and DQB1* and encoding two different HLA DQ heterodimers is the prerequisite. We aimed to determine the frequency of these haplotypes in CVID patients with suspected CD. Furthermore, we report on autoimmune manifestations and the lymphocyte phenotype in these patients.

Methods

By retrospective analysis data on gastrointestinal symptoms, diet, concurrent autoimmune diseases, and routine laboratory values were collected. CVID patients were classified according to their B-cell phenotype. Expression of HLA-DQA1* and HLA-DQB1* alleles were determined by genetic analysis.

Results

Twenty out of 250 CVID patients presented with a clinical phenotype resembling celiac disease. Four (20 %) out of these CVID patients carried the CD-associated HLA DQ2.5 or DQ8 heterodimer, while HLA DQ2.5 was present in 100 % of a CD control cohort. Gluten-free diet (GFD) resulted in a clinical and histological response in two out of four patients with HLA high-risk alleles for CD. The response could not be assessed in the remaining two patients, as these patients did not adhere sufficiently long to GFD. The percentage of autoimmune manifestations other than CD was high (50 %) in CVID patients presenting with a CD-like enteropathy, and most of these patients had an expansion of B-cells with low expression of CD21 (CD21low B-cells).

Conclusions

In CVID patients with suspected celiac disease typing of the HLA loci DQA1 and DQB1 can help to identify those that have a genetic susceptibility for CD. In CVID patients with a celiac-like phenotype but negative for CD-associated HLA-DQ markers, an autoimmune enteropathy (AIE) as part of an extended autoimmune dysregulation needs to be considered. This has important implications for further diagnostics and therapy of these patients.

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Acknowledgments

We thank the patients participating in this study. This study was supported by the Federal Ministry of Education and Research (BMBF 01EO 0803). The authors are responsible for the contents of this publication.

Conflict of Interest

The authors have to conflict of interest to declare.

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Authors and Affiliations

  1. Department of Rheumatology and Clinical Immunology, University Medical Center Freiburg, Freiburg, Germany

    Nils Venhoff, Ulrich Salzer, Nora M. Effelsberg, Florian Kollert, Reinhard E. Voll, Klaus Warnatz & Jens Thiel

  2. Department of Transfusion Medicine, University Medical Center Freiburg, Freiburg, Germany

    Florian Emmerich, Corinna Koehn & Sibyll Driever

  3. Department of Gastroenterology and Hepatology, University Medical Center Freiburg, Freiburg, Germany

    Michaela Neagu & Wolfgang Kreisel

  4. Center for Chronic Immunodeficiency, University Medical Center Freiburg, Freiburg, Germany

    Ulrich Salzer, Marta Rizzi, Sigune Goldacker, Reinhard E. Voll & Klaus Warnatz

  5. University Medical Center Freiburg, Hugstetterstrasse 55, 79106, Freiburg, Germany

    Nils Venhoff

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  1. Nils Venhoff
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  2. Florian Emmerich
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Correspondence to Nils Venhoff.

Additional information

Florian Emmerich and Michaela Neagu contributed equally

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Venhoff, N., Emmerich, F., Neagu, M. et al. The Role of HLA DQ2 and DQ8 in Dissecting Celiac-Like Disease in Common Variable Immunodeficiency. J Clin Immunol 33, 909–916 (2013). https://doi.org/10.1007/s10875-013-9892-3

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  • DOI: https://doi.org/10.1007/s10875-013-9892-3

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