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Molecular characterisation of methicillin-susceptible and methicillin-resistant Staphylococcus aureus in inpatients and outpatients in Bosnia and Herzegovina

Molekulare Charakterisierung Methacillin-sensibler und Methacillin-resistenter Isolate von Staphylococcus aureus bei stationären und ambulant behandelten Patienten in Bosnien und Herzegovina

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Summary

The aim of this study was to investigate the genetic background of methicillin-susceptible (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) obtained from clinical specimens of inpatients and outpatients. Methicillin resistance was confirmed by the presence of the mecA gene by PCR. The genetic characterisation was performed using spa typing and the algorithm based upon repeat pattern (BURP). Staphylococcus aureus was isolated from 68 and 79 inpatient and outpatient samples, 31 (46 %) and 14 (18 %) of which were MRSA, respectively. Among 37 inpatients and 65 outpatients with MSSA, 22 and 38 spa types were clustered into seven and eight spa-CCs, respectively. The main MSSA spa-CC of inpatients and outpatients was spa-CC015 (multilocus sequence typing (MLST) CC45). Most MRSA were associated with spa-CC355/595 (MLST CC152). MRSA-related background was found in 32 % of inpatients and 43 % of outpatients with MSSA, suggesting that MRSA did not arise from predominant MSSA clones.

Zusammenfassung

Das Ziel der vorliegenden Arbeit war es, den genetischen Hintergrund von Methacillin-sensiblen (MSSA) und Methacillin-resistenten Staphylococcus aureus (MRSA), die aus verschiedenen klinischen Proben bei stationär und ambulant behandelten Patienten gewonnen wurden, zu erforschen. Die Methacillin-Resistenz wurde durch die Anwesendheit von Meca-Genen PCR-DM bestätigt. Die genetische Charakterisierung erfolgte mittels SPA Typisierung und BURP. Staphylococcus aureus wurde aus 68 und 79 Proben der stationär bzw. ambulant behandelten Patienten isoliert, 31 (46 %) bzw. 14 (18 %) davon waren MRSA. Bei 37 stationären und 65 ambulanten Patienten mit MSSA wurden 22 bzw. 38 SPA-Typen in sieben bzw. acht Haupt-SPA-Clonal Complexes (CC) klassifiziert. Haupt-MSSA SPA-CC war CC015 (MLST CC45), bei 16 % der stationären bzw. 21 % der ambulanten Patienten. Die meisten der MRSA Isolate waren SPA-CC 355/595 (MLST CC152). Bei 32 % der stationären und 43 % der ambulanten Patienten wiesen die MSSA Isolate einen MRSA-Hintergrund auf; dies weist darauf hin, dass die MRSA nicht aus den dominanten MSSA-Klonen hervorgegangen sind.

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Conflict of interest

The authors declare no conflict of interest related to this article.

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Authors and Affiliations

  1. Department for Laboratory Diagnostics, Cantonal Public Health Institute Zenica, Fra Ivana Jukica 2, 72000, Zenica, Bosnia and Herzegovina

    Selma Uzunović-Kamberović & Amir Ibrahimagić

  2. Faculty for Health Care and Nursing, University “VITEZ” Travnik, Travnik, Bosnia and Herzegovina

    Selma Uzunović-Kamberović

  3. Department for Medical Microbiology, The School for Public Health and Primary Care (CAPHRI), Maastricht University Medical Center, Mastricht, The Netherlands

    Michelle I. A. Rijnders & Ellen E. Stobberingh

  4. Microbiology Department, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia

    Farah Kamberović

  5. Department for Biostatistics, Medical Informatics, and Researches in Medicine, Faculty of Medicine, University of Belgrade, Belgrade, Serbia

    Tatjana Ille

Authors
  1. Selma Uzunović-Kamberović
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  2. Michelle I. A. Rijnders
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  3. Ellen E. Stobberingh
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  4. Amir Ibrahimagić
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  5. Farah Kamberović
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  6. Tatjana Ille
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Corresponding author

Correspondence to Selma Uzunović-Kamberović.

Additional information

This paper was partly presented by Selma Uzunović-Kamberović, Michelle I. A. Rijnders, Ellen E. Stobberingh, Amir Ibrahimagić, Farah Kamberović, Jasmin Dizdarevic, Tatjana Ille and Salih Tandir. Molecular characterisation of clinical (inpatients/outpatients) and colonising methicillin-susceptible and methicillin-resistant Staphylococcus aureus in Bosnia and Herzegovina. The 4th Euro-Asian Congress of Infectious Diseases, Sarajevo/B&H, June 1–5, 2011. In: Karahasan A, Gunaydin M, AhmetagiĆ S, TihiĆ N, Hosoglu S, Leblebicioglu, eds. Abstract book. Bilimsel Tip Yayinevi, Ankara, 2011. Abstract No. 061, p. 175.

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Uzunović-Kamberović, S., Rijnders, M., Stobberingh, E. et al. Molecular characterisation of methicillin-susceptible and methicillin-resistant Staphylococcus aureus in inpatients and outpatients in Bosnia and Herzegovina. Wien Med Wochenschr 163, 13–20 (2013). https://doi.org/10.1007/s10354-012-0142-8

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  • DOI: https://doi.org/10.1007/s10354-012-0142-8

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