Skip to main content

Advertisement

SpringerLink
Log in

Healing process after surgical treatment with scalpel, electrocautery and laser radiation: histomorphologic and histomorphometric analysis

  • Original Article
  • Published:
Lasers in Medical Science Aims and scope Submit manuscript

Abstract

The aim of this study was to evaluate and compare the healing process after surgical treatment of chemically induced lesions in the lateral edge of tongue of hamsters performed with scalpel, electrocautery, carbon dioxide (CO2) laser radiation or neodymium:yttrium–aluminum–garnet (Nd:YAG) laser radiation. Eighty hamsters of both sexes were used and examined at postoperative days 7, 14, 21 and 28 by histological and histomorphometric analysis of the skeletal muscle fibers. In the histological analysis it was observed that the dynamics of the healing process was faster in the group treated by scalpel than in the other groups. The histomorphometric observation of the skeletal muscle fibers was submitted to one-way analysis of variance (ANOVA) and Tukey’s multiple comparison test, with a level of significance set at P < 0.05, which showed that the amount of skeletal muscle fiber formed had significantly increased in the group treated by scalpel in comparison with that in the groups treated by electrocautery (P < 0.01), CO2 laser irradiation (P < 0.001) and Nd:YAG laser irradiation (P < 0.01) on the 14th postoperative day. A gradual increase in skeletal muscle fibers formed during the healing process was observed in all groups. When the laser irradiated groups were compared, it was possible to conclude that tissue organization and vascularization were faster and more intense in the Nd:YAG laser irradiated group than in the CO2 laser irradiated group.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Cotran RS, Kumar V, Robbins SL (1999) Robbins: Patologia estrutural e funcional, 6th edn. Guanabara Koogan, Rio de Janeiro

    Google Scholar 

  2. Li J, Chen J, Kirsner R (2007) Pathophysiology of acute wound healing. Clin Dermatol 25:9–18

    Article  CAS  PubMed  Google Scholar 

  3. Romanos G, Siar CH, Ng K, Toh CG (1999) A preliminary study of healing of superpulsed carbon dioxide laser incisions in the hard palate of monkeys. Lasers Surg Med 24:368–374

    Article  CAS  PubMed  Google Scholar 

  4. Morosolli ARC, Schubert MM, Niccoli-Filho W (2006) Surgical treatment of erythroleukoplakia in lower lip with carbon dioxide laser radiation. Lasers Med Sci 21:181–184

    Article  Google Scholar 

  5. Deppe H, Horch HH (2007) Current status of laser applications in oral cranio-maxillofacial surgery. Med Laser Appl 22:39–42

    Article  Google Scholar 

  6. Rossmann JA, Gottlieb S, Koudelka BM, McQuade MJ (1987) Effects of CO2 laser irradiation on gingiva. J Periodontol 58:423–425

    CAS  PubMed  Google Scholar 

  7. Myers TD, Myers WD, Stone RM (1989) First soft tissue study utilizing a pulsed Nd:YAG dental laser. North West Dent 68:14–17

    CAS  Google Scholar 

  8. Romanos GE, Pelekanos S, Strub JR (1995) Effects of Nd:YAG laser on wound healing processes: clinical and immunohistochemical findings in rat skin. Lasers Surg Med 16:368–379

    Article  CAS  PubMed  Google Scholar 

  9. Frame JW (2003) Recent progress with the CO2 laser in oral surgery. Int Congr Ser 1248:3–7

    Article  Google Scholar 

  10. Cantatore J, Kriegel D (2004) Laser surgery: an approach to the pediatric patient. J Am Dermatol 50:165–184

    Article  Google Scholar 

  11. Sinha VK, Gallagher LA (2003) Effects of steel scalpel, ultrasonic scalpel, CO2 laser, and monopolar and bipolar electrosurgery on wound healing in guinea pig oral mucosa. Laryngoscope 113:228–236

    Article  PubMed  Google Scholar 

  12. Camacho-Alonso F, López-Jornet P (2007) Clinical-pathologic study of the healing of wounds provoked on the dorso-lingual mucosa in 186 albino rats. Otolaryngol Head Neck Surg 136:119–124

    Article  PubMed  Google Scholar 

  13. Gomes MF, Anjos MJS, Nogueira TO, Catanzaro-Guimarães SA (2002) Autogenous demineralized dentin matrix for tissue engineering applications: radiographic and histomorphometric studies. Int J Oral Maxillofac Implants 17:488–497

    PubMed  Google Scholar 

  14. Masaki M, Obara K, Suzuki S, Orikasa K, Mitsuhashi H, Iwasaki K, et al (1990) The destructive effects of sclerosant ethanolamine oleate on mammalian vessel endothelium. Gastroenterol Jpn 25:230–235

    CAS  PubMed  Google Scholar 

  15. Fisher SE, Frame JW, Browne RM, Trante RM (1983) A comparative histological study of wound healing following CO2 laser and conventional surgical excision of canine buccal mucosa. Arch Oral Biol 28:287–291

    Article  CAS  PubMed  Google Scholar 

  16. Pogrel MA (1989) The carbon dioxide laser in soft tissue preprosthetic surgery. J Prosthetic Dent 61:203–208

    Article  CAS  Google Scholar 

  17. Luomanen M, Lehto VP, Meurman JH (1988) Myofibroblasts in healing laser wounds of rat tongue mucosa. Arch Oral Biol 33:17–23

    Article  CAS  PubMed  Google Scholar 

  18. Walsh LJ (1996) Pulpal temperature changes during low-power had-tissue CO2 laser procedures. Braz Dent J 7:5–11

    CAS  PubMed  Google Scholar 

  19. Friesen LR, Cobb CM, Rappley JW, Forgas-Brockman L, Spencer P (1999) Laser irradiation of bone: II. Healing response following treatment by CO2 and Nd:YAG lasers. J Periodontol 70:75–83

    Article  CAS  PubMed  Google Scholar 

  20. Paes-Junior TA, Niccoli-Filho W (2001) Clinical comparison between conventional suture and vaporization with carbon dioxide laser in rat’s skin. J Clin Laser Med Surg 19:319–324

    Article  CAS  PubMed  Google Scholar 

  21. Thuaksuban N, Nuntanaranont T (2003) A comparative study of postoperative pain using carbon dioxide laser and scalpel in maxillofacial soft tissue surgery. Int Congress Series 1248:377–381

    Article  Google Scholar 

  22. Horch HH, Deppe H (2005) New aspects of lasers in oral and craniomaxillofacial surgery. Med Laser Appl 20:7–11

    Article  Google Scholar 

  23. Evrard L, Nammour S, Dourov N (1996) Scanning electron microscopic and immunocytochemical studies of contraction during secondary CO2 laser wound healing in rat tongue mucosa. J Oral Pathol Med 25:72–77

    Article  CAS  PubMed  Google Scholar 

  24. Kardos TB, Holt T, Ferguson MM (1989) Histological evaluation of the effect of a miniature carbon dioxide laser on oral mucosa. Int J Maxillofac Surg 18:117–120

    Article  CAS  Google Scholar 

  25. Fortune DS, Huang S, Soto J, Pennington B, Ossof RH, Reinisch L (1998) Effect of pulse duration on wound healing using a CO2 laser. Laryngoscope 108:843–848

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

Financial support for this study was received from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (protocol number 141535/2006-7).

Author information

Authors and Affiliations

  1. Stomatologic Clinic, School of Dentistry, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil

    Aline Rose Cantarelli Morosolli

  2. Department of Radiology, School of Dentistry, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil

    Elaine Bauer Veeck

  3. Academic Group of Studies and Research with Laser in Dentistry and Department of Biosciences and Oral Diagnosis, Dental School, Sao Paulo State University, (UNESP), Sao Jose dos Campos, Sao Paulo, Brazil

    Walter Niccoli-Filho

  4. Department of Biosciences and Oral Diagnosis, São José dos Campos Dental School, São Paulo State University (UNESP) and Bioscience Center for Special Health Care (CEBAPE-UNESP), São José dos Campos Dental School, UNESP, Sao Jose dos Campos, Sao Paulo, Brazil

    Mônica Fernandes Gomes

  5. Bioscience Center for Special Health Care (CEBAPE-UNESP), São José dos Campos Dental School, UNESP, Sao Jose dos Campos, Sao Paulo, Brazil

    Maria das Graças V. Goulart

  6. Rua Demétrio Ribeiro, 1017/707, Centro, Porto Alegre, Rio Grande do Sul, 90010-311, Brazil

    Aline Rose Cantarelli Morosolli

Authors
  1. Aline Rose Cantarelli Morosolli
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Elaine Bauer Veeck
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Walter Niccoli-Filho
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mônica Fernandes Gomes
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Maria das Graças V. Goulart
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Aline Rose Cantarelli Morosolli.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Morosolli, A.R.C., Veeck, E.B., Niccoli-Filho, W. et al. Healing process after surgical treatment with scalpel, electrocautery and laser radiation: histomorphologic and histomorphometric analysis. Lasers Med Sci 25, 93–100 (2010). https://doi.org/10.1007/s10103-009-0674-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10103-009-0674-3

Keywords

Search

Navigation