Abstract
The purpose of this study was to investigate methicillin-resistant Staphylococcus aureus (MRSA) isolates with antibiotic resistance restricted to beta-lactam antibiotics and variable resistance to fusidic acid for the presence of Panton-Valentine leukocidin (PVL) genes. Our data show that the selected resistance pattern is found rarely among MRSA isolates in our hospital, but it appears that this phenotype consistent with typical community-acquired (ca) MRSA is indicative of PVL-positive MRSA.
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Deurenberg RH, Vink C, Kalenic S, Friedrich AW, Bruggeman CA, Stobberingh EE (2007) The molecular evolution of methicillin-resistant Staphylococcus aureus. Clin Microbiol Infect 13(3):222–235. doi:10.1111/j.1469-0691.2006.01573.x
Boyle-Vavra S, Daum RS (2007) Community-acquired methicillin-resistant Staphylococcus aureus: the role of Panton-Valentine leukocidin. Lab Invest 87(1):3–9. doi:10.1038/labinvest.3700501
Diederen BM, Kluytmans JA (2006) The emergence of infections with community-associated methicillin resistant Staphylococcus aureus. J Infect 52(3):157–168. doi:10.1016/j.jinf.2005.09.001
Tiemersma EW, Bronzwaer SL, Lyytikäinen O, Degener JE, Schrijnemakers P, Bruinsma N, Monen J, Witte W, Grundman H; European Antimicrobial Resistance Surveillance System Participants (2004) Methicillin-resistant Staphylococcus aureus in Europe, 1999–2002. Emerg Infect Dis 10(9):1627–1634
Raab U, Kahlau D, Wagenlehner F, Reischl U, Ehrenstein V, Lehn N, Holler C, Linde HJ (2006) Prevalence of and risk factors for carriage of Panton-Valentine leukocidin-positive methicillin-resistant Staphylococcus aureus among residents and staff of a German nursing home. Infect Control Hosp Epidemiol 27(2):208–211. doi:10.1086/500629
Chizzali-Bonfadin C, Adlassnig KP, Koller W (1995) MONI: an intelligent database and monitoring system for surveillance of nosocomial infections. Medinfo 8(Pt 2):1684
National Committee for Clinical Laboratory Standards (NCCLS) (1999) Performance standards for antimicrobial susceptibility testing: 9th informational supplement, M100-S9. NCCLS, Wayne, PA, USA
Willinger B, Rotter M (1991) Resistance of staphylococci to fusidic acid in the Vienna area and interpretative criteria for results of susceptibility tests. Hyg Med 16:25–28
Domann E, Hossain H, Füssle R, Chakraborty T (2000) Rapid and reliable detection of multiresistant Staphylococcus aureus (MRSA) by multiplex PCR. Dtsch Med Wochenschr 125(20):613–618. doi:10.1055/s-2007-1024385
Lina G, Piémont Y, Godail-Gamot F, Bes M, Peter MO, Gauduchon V, Vandenesch F, Etienne J (1999) Involvement of Panton-Valentine leukocidin-producing Staphylococcus aureus in primary skin infections and pneumonia. Clin Infect Dis 29(5):1128–1132. doi:10.1086/313461
Strommenger B, Kettlitz C, Weniger T, Harmsen D, Friedrich AW, Witte W (2006) Assignment of Staphylococcus isolates to groups by spa typing, SmaI macrorestriction analysis, and multilocus sequence typing. J Clin Microbiol 44(7):2533–2540. doi:10.1128/JCM.00420-06
Harmsen D, Claus H, Witte W, Rothgänger J, Claus H, Turnwald D, Vogel U (2003) Typing of methicillin-resistant Staphylococcus aureus in a university hospital setting by using novel software for spa repeat determination and database management. J Clin Microbiol 41(12):5442–5448. doi:10.1128/JCM.41.12.5442-5448.2003
Okuma K, Iwakawa K, Turnidge JD, Grubb WB, Bell JM, O’Brien FG, Coombs GW, Pearman JW, Tenover FC, Kapi M, Tiensasitorn C, Ito T, Hiramatsu K (2002) Dissemination of new methicillin-resistant Staphylococcus aureus clones in the community. J Clin Microbiol 40(11):4289–4294. doi:10.1128/JCM.40.11.4289-4294.2002
Friedrich AW, Witte W, Harmsen D, de Lencastre H, Hryniewicz W, Scheres J, Westh H; SeqNet.org participants (2006) SeqNet.org: a European laboratory network for sequence-based typing of microbial pathogens. Euro Surveill 11(1):E060112.4
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Bauer, C.C., Apfalter, P., Daxboeck, F. et al. Prevalence of Panton-Valentine leukocidin genes in methicillin-resistant Staphylococcus aureus isolates phenotypically consistent with community-acquired MRSA, 1999–2007, Vienna General Hospital. Eur J Clin Microbiol Infect Dis 28, 909–912 (2009). https://doi.org/10.1007/s10096-009-0721-9
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DOI: https://doi.org/10.1007/s10096-009-0721-9