Abstract
Background
This study investigated the effect of intravenous administration of verapamil in prevention of the injury caused by free oxygen radicals generated in a rabbit retroperitoneoscopic donor nephrectomy model.
Methods
Twenty-four adult New Zealand rabbits were divided into four groups. In group I, balloon dissection of the left retroperitoneal space was performed. In group II, CO2 at 10 mmHg was applied for 3 hours after the balloon dissection. In group III, laparotomy was performed, the left renal pedicle was clamped for 3 min, and the clamp was removed 5 min before nephrectomy. In group IV, 2 min before the attempt 0.2 mg/kg verapamil was given intravenously, and the same procedure was employed as in group III. Nephrectomy was performed after each experiment. The concentrations of malonyl dialdehyde (MDA), reduced glutathione (GSH), and protein carbonyl content were measured in renal tissue samples as markers of oxidative stress.
Results
Pneumoretroperitoneum (Prp) promoted oxidative stress in renal tissues, with an increase of MDA and protein carbonyl content. The verapamil- pretreated group (group IV) showed statistical significantly lower values of MDA and protein carbonyl content when compared with group II and III (p < 0.05), whereas tissue GSH concentrations were unchanged in all groups.
Conclusions
Our study showed that Prp causes increased oxidative stress in renal tissue. Warm ischemia lasting 3 min did not exert an additive effect on Prp-associated oxidative stress. Verapamil reduces the oxidative stress markers caused by Prp.
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References
G Akbulut M Serteser C Polat T Koken F Aktepe S Yilmaz C Gokce O Gokce (2002) ArticleTitleChanges in tissue oxidative stress markers in an experimental model of laparoscopic donor nephrectomy. Transplantation 27 1768–1772 Occurrence Handle10.1097/00007890-200212270-00022
SP Andreoli (1991) ArticleTitleReactive oxygen molecules, oxidant injury and renal disease. Pediatr Nephrol 5 733 Occurrence Handle1:STN:280:By2C3Mfmt1w%3D Occurrence Handle1662982
E Beutler MJ Robson E Buttenwieser (1957) ArticleTitleThe glutathione instability of drug sensitive red cells. J Lab Clin Med 49 84–88 Occurrence Handle1:STN:280:CyiD3s7nslA%3D Occurrence Handle13385579
P Di Simplicio KH Cheeseman TF Slater (1991) ArticleTitleThe reactivity of the SH group of bovine serum albumin with free radicals. Free Rad Res Commun 14 253 Occurrence Handle1:CAS:528:DyaK3MXltVyrsLk%3D
HK Elkadi AH Mardan DD Nghiem JH Southard (1989) ArticleTitleThe role of calcium antagonists in the management of renal warm ischemia. J Urol 141 974–980 Occurrence Handle1:STN:280:BiaC2sfpsVQ%3D Occurrence Handle2926910
O Erdogan S Yildiz A Basaran A Demirbas A Yesilkaya (2001) ArticleTitleEffect of intraportal verapamil infusion on hepatic ischemia-reperfusion injury. Pol J Pharmacol 53 137–141 Occurrence Handle1:CAS:528:DC%2BD3MXms1Sqsbg%3D Occurrence Handle11787953
DD Gaur (1992) ArticleTitleLaparoscopic operative retroperitoneoscopy: use of a new device. J Urol 148 1137–1139 Occurrence Handle1:STN:280:ByyD387kt1c%3D Occurrence Handle1404623
IS Gill RV Clayman DM Albala Y Aso AW Chiu S Das JF Donovan GJ Fuchs DD Gaur H Go LG Gomella MT Grune LM Harewood G Janetschek PM Knapp EM McDougall SY Nakada GM Preminger P Puppo JJ Rassweiler PL Royce R Thomas DA Urban HN Winfield (1998) ArticleTitleRetroperitoneal and pelvic extraperitoneal laparoscopy; an international perspective. Urology 52 566–571 Occurrence Handle10.1016/S0090-4295(98)00314-8 Occurrence Handle1:STN:280:DyaK1cvjsVSgtA%3D%3D Occurrence Handle9763072
C Guler M Demirbas M Samli T Koken C. Dincel (2003) ArticleTitleThe effect of retroperitoneoscopic donor nephrectomy on tissue oxidative stress markers in an experimental rabbit retroperitoneum model. J Endourol 17 935–939 Occurrence Handle10.1089/089277903772036325 Occurrence Handle14744367
C Guler M Sade Z Kirkalı (1998) ArticleTitleRenal effects of carbon-dioxide insufflation in rabbit pneumoretroperitoneum model. J Endourol 12 367–370 Occurrence Handle1:STN:280:DyaK1czptlSjtQ%3D%3D Occurrence Handle9726406
B Halliwell (1991) ArticleTitleReactive oxygen species in living systems: source, biochemistry and role in human disease. Am J Med 91 14S Occurrence Handle10.1016/0002-9343(91)90279-7 Occurrence Handle1:CAS:528:DyaK38Xitlartr0%3D
AK Hemal NP Gupta SN Wadhwa A Goel R Kumar (2001) ArticleTitleRetroperitoneoscopic nephrectomy and nephroureterectomy for benign nonfunctioning kidneys: a single-center experience. Urology 57 644–649 Occurrence Handle10.1016/S0090-4295(00)01059-1 Occurrence Handle1:STN:280:DC%2BD3M3ms1Wruw%3D%3D Occurrence Handle11306369
RL Levine D Garland CN Oliver A Amici I Climent AG Lenz BW Ahn S Shaltiel ER Stadtman (1990) ArticleTitleDetermination of carbonyl content in oxidatively modified proteins. Methods Enzymol 186 464 Occurrence Handle10.1016/0076-6879(90)86141-H Occurrence Handle1:CAS:528:DyaK3MXksVGhtLY%3D Occurrence Handle1978225
H Okhawa N Ohishi K Yagi (1979) ArticleTitleAssay for lipid peroxidase in animal tissues by thiobarbituric acid reaction. Anal Biochem 95 351 Occurrence Handle1:CAS:528:DyaE1MXksFaisbk%3D Occurrence Handle36810
H Rabb YM O’Meara P Maderna P Coleman HR Brady (1997) ArticleTitleLeucocytes, cell adhesion molecules and ischemic acute renal failure. Kidney Int 51 1463 Occurrence Handle1:CAS:528:DyaK2sXjsFSht7g%3D Occurrence Handle9150459
JI Shapiro C Cheung A Itabashi L Chan RW Schrier (1985) ArticleTitleThe effect of verapamil on renal function after warm and cold ischemia in the isolated perfused rat kidney. Transplantation 40 596–600 Occurrence Handle1:CAS:528:DyaL2MXmtFagsLo%3D Occurrence Handle4071610
H Sies (1991) ArticleTitleOxidative stress: oxidants and antioxidants. Exp Physiol 82 291
ER Stadtman BS Berlett (1997) Free radical mediated modification of proteins. KB Wallace (Eds) Free radical toxicology Taylor & Francis 71–87
ER Stadtman BS Berlett (1998) ArticleTitleReactive oxygen-mediated protein oxidation in aging and disease. Drug Metab Rev 30 225–243 Occurrence Handle1:CAS:528:DyaK1cXjsF2qsLw%3D Occurrence Handle9606602
M Takada KC Nadeau GD Shaw KA Marquette NL Tilney (1997) ArticleTitleThe cytokine-adhesions molecule cascade in ischemia/reperfusion injury of the rat kidney. J Clin Invest 99 2682 Occurrence Handle1:CAS:528:DyaK2sXjs1Kmtrs%3D Occurrence Handle9169498
M Uchiyama M Mihara (1978) ArticleTitleDetermination of malondialdehyde precursor in tissues by the thiobarbituric acid test. Anal Biochem 86 271 Occurrence Handle1:STN:280:CSeC1crgtVc%3D Occurrence Handle655387
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This study was conducted at Konya Selçuk University Animal Research Laboratories
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Demirbas, M., Guler, C., Samlı, M. et al. The effect of verapamil on the prevention of ischemia/reperfusion injury in the experimental retroperitoneoscopic donor nephrectomy model. Surg Endosc 18, 1272–1275 (2004). https://doi.org/10.1007/s00464-003-9145-2
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DOI: https://doi.org/10.1007/s00464-003-9145-2