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Absence of caveolin-1 expression in carcinoma-associated fibroblasts of invasive micropapillary carcinoma of the breast predicts poor patient outcome

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Abstract

Caveolin-1 (Cav-1) expression in stromal carcinoma-associated fibroblasts (CAFs) has been associated with tumor progression and clinical outcome. This study was undertaken to assess its prognostic significance in invasive micropapillary carcinoma of the breast (IMPC), a tumor with abundant stromal CAFs and a high tendency for nodal metastasis and poor outcome. Cav-1 expression was studied by immunohistochemistry in a group of 86 cases of IMPC along with a control group of 105 cases of invasive ductal carcinoma, not otherwise specified (IDC-NOS). Our results indicate that absence of Cav-1 expression in CAFs of IMPC is more common than in IDC-NOS (57 %, 49/86 vs. 36 %, 38/105). The absence of expression was associated with larger tumor size and higher lymph node stage (P < 0.05) of IMPC. Univariate analysis suggested absence of Cav-1 in CAFs to be a candidate independent predictor of reduced progression-free survival (PFS) (HR = 3.945, 95 % CI = 1.717–9.063, P = 0.001), which was confirmed by multivariable analysis (P = 0.018). In patients with IMPC spreading to local lymph nodes, loss of stromal Cav-1 predicted a fourfold increase in risk for shortened PFS. In contrast, no significant difference of tumor epithelial Cav-1 expression was found between IMPC and IDC-NOS, and the expression of tumor Cav-1 was not significantly associated with the prognosis of patients with IMPC. Absence of Cav-1 expression in CAFs is a strong prognostic factor for IMPC patients, and it may further subgroup the patients with lymph node metastasis to guide clinical management.

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Acknowledgments

This work was financially supported by National Natural Science Foundation of China (Grant No. 30930038, No. 81202101 and No. 81302292) and Innovation Funding for graduate of Tianjin Medical University, third phase of the 211 Project for Higher Education (Grant No. 2010GSI07).

Conflict of interest

We declare that we have no conflict of interest.

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Authors and Affiliations

  1. Department of Breast Cancer Pathology and Research Laboratory, Key Laboratory of Breast Cancer Prevention and Therapy (Tianjin Medical University, Ministry of Education), Key Laboratory of Cancer Prevention and Therapy, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center of Cancer, Huan hu xi Road, Tiyuan Bei, Hexi District, Tianjin, 300060, China

    Meijing Ren, Fangfang Liu, Yufen Zhu, Yaqing Li, Ronggang Lang, Yu Fan, Feng Gu & Li Fu

  2. Department of Pathology and Laboratory Medicine, Temple University Hospital, Philadelphia, PA, USA

    Xinmin Zhang

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  1. Meijing Ren
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  2. Fangfang Liu
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  6. Yu Fan
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  7. Feng Gu
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  8. Xinmin Zhang
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  9. Li Fu
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Correspondence to Li Fu.

Additional information

M. Ren and F. Liu contributed equally to this study and should be regarded as joint first authors.

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Ren, M., Liu, F., Zhu, Y. et al. Absence of caveolin-1 expression in carcinoma-associated fibroblasts of invasive micropapillary carcinoma of the breast predicts poor patient outcome. Virchows Arch 465, 291–298 (2014). https://doi.org/10.1007/s00428-014-1614-6

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  • DOI: https://doi.org/10.1007/s00428-014-1614-6

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