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01.12.2013 | original article | Ausgabe 6/2013

European Surgery 6/2013

Impact of pneumoperitoneum on collagen I expression in vitro

European Surgery > Ausgabe 6/2013
MD T. F. Ulmer, Dr. M. Binnebösel, A. Mossdorf, Prof. Dr. U. P. Neumann, Dr. R. Rosch



There is little and contradictory data about the impact of laparoscopy on wound healing. In this experimental study, gas-related effects of pneumoperitoneum on collagen I mRNA synthesis were analysed separately in fibroblast cultures in vitro.


3T3 mouse fibroblasts were incubated for 1 h with either CO2 or helium (gas flow: 1 l/min, pressure: 12 mmHg). Control fibroblasts were exposed to room air for 1 h. Hereafter, expression of collagen type IA1 was analysed by real-time PCR.


Expression of collagen type IA1 analysed was 0.7 times lower after incubation with CO2 when compared with controls. After helium insufflation, collagen type IA1 expression showed a 1.1-fold increase.


The observed gas-related influence on collagen synthesis might give evidence of a potential effect of laparoscopy on intra-abdominal wound healing.

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