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01.12.2013 | original article | Ausgabe 6/2013

European Surgery 6/2013

Impact of pneumoperitoneum on collagen I expression in vitro

Zeitschrift:
European Surgery > Ausgabe 6/2013
Autoren:
MD T. F. Ulmer, Dr. M. Binnebösel, A. Mossdorf, Prof. Dr. U. P. Neumann, Dr. R. Rosch

Summary

Background

There is little and contradictory data about the impact of laparoscopy on wound healing. In this experimental study, gas-related effects of pneumoperitoneum on collagen I mRNA synthesis were analysed separately in fibroblast cultures in vitro.

Methods

3T3 mouse fibroblasts were incubated for 1 h with either CO2 or helium (gas flow: 1 l/min, pressure: 12 mmHg). Control fibroblasts were exposed to room air for 1 h. Hereafter, expression of collagen type IA1 was analysed by real-time PCR.

Results

Expression of collagen type IA1 analysed was 0.7 times lower after incubation with CO2 when compared with controls. After helium insufflation, collagen type IA1 expression showed a 1.1-fold increase.

Conclusions

The observed gas-related influence on collagen synthesis might give evidence of a potential effect of laparoscopy on intra-abdominal wound healing.

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