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Erschienen in: Wiener klinische Wochenschrift 5/2015

01.12.2015 | original article

HUIEC, Human intestinal epithelial cell line with differentiated properties: process of isolation and characterisation

verfasst von: Lidija Gradisnik, Martin Trapecar, Marjan Slak Rupnik, MDMSc Tomaz Velnar

Erschienen in: Wiener klinische Wochenschrift | Sonderheft 5/2015

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Summary

The intestinal epithelium is composed of diverse cell types, most abundant being the enterocytes. Among other functions, they maintain the intestinal barrier and play a critical role in the absorption of nutrients, drugs and toxins. This study describes the development and characterization of human intestinal epithelial cells (HUIEC), a spontaneously arising cell line established by selective trypsinization and cloning of the intestinal epithelium, resulting in a uniform population of highly epithelial cells with a strong growth potential.
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Metadaten
Titel
HUIEC, Human intestinal epithelial cell line with differentiated properties: process of isolation and characterisation
verfasst von
Lidija Gradisnik
Martin Trapecar
Marjan Slak Rupnik
MDMSc Tomaz Velnar
Publikationsdatum
01.12.2015
Verlag
Springer Vienna
Erschienen in
Wiener klinische Wochenschrift / Ausgabe Sonderheft 5/2015
Print ISSN: 0043-5325
Elektronische ISSN: 1613-7671
DOI
https://doi.org/10.1007/s00508-015-0771-1