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01.12.2015 | original article | Sonderheft 5/2015

Wiener klinische Wochenschrift 5/2015

HUIEC, Human intestinal epithelial cell line with differentiated properties: process of isolation and characterisation

Zeitschrift:
Wiener klinische Wochenschrift > Sonderheft 5/2015
Autoren:
Lidija Gradisnik, Martin Trapecar, Marjan Slak Rupnik, MDMSc Tomaz Velnar

Summary

The intestinal epithelium is composed of diverse cell types, most abundant being the enterocytes. Among other functions, they maintain the intestinal barrier and play a critical role in the absorption of nutrients, drugs and toxins. This study describes the development and characterization of human intestinal epithelial cells (HUIEC), a spontaneously arising cell line established by selective trypsinization and cloning of the intestinal epithelium, resulting in a uniform population of highly epithelial cells with a strong growth potential.

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