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Erschienen in: Wiener klinische Wochenschrift 23-24/2015

01.12.2015 | original article

Evaluation of the immunochromatographic (Leptocheck) test for detection of specific antibodies against leptospires

verfasst von: univ. dipl. mikr. Daša Podgoršek, Tjaša Cerar, Mateja Logar, Gorazd Lešničar, Tatjana Remec, Zvonko Baklan, Emil Pal, Eva Ružić-Sabljić

Erschienen in: Wiener klinische Wochenschrift | Ausgabe 23-24/2015

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Summary

Background

Leptospirosis is a febrile worldwide zoonosis. Routine diagnosis of leptospiral infection is based on demonstration of specific antibodies with serological tests. Performance of the reference serological test, the microscopic agglutination test (MAT), requires significant expertise. The aim of our study was to find out if leptospiral infection can be proven with simple, rapid, commercially available immunochromatographic Leptocheck test in order to introduce it for the first level diagnosis in emergency cases with less specialized laboratory staff.

Methods

In all, 590 serum samples of patients with clinical manifestations suggestive of leptospirosis were collected and tested with MAT and Leptocheck test. For confirmation of the results some other diagnostic methods such as polymerase chain reaction (PCR) and Leptospira isolation were performed.

Results

Results of both serological tests were consistent in 576/590 (97.63 %) cases but Leptocheck gave more positive results in comparison to MAT (36 and 12, respectively) at first patient’s testing. Following up the patient, MAT became positive in majority of Leptocheck positive patients at first visit. Leptospiral DNA was detected in nine blood and six urine samples belonging to thirteen different patients while only two samples were culture positive.

Conclusion

In comparison with serological tests, PCR and culture have low sensitivity. According to our findings we conclude that Leptocheck test can prove leptospiral infection and could be used for rapid diagnosis of leptospirosis, later the sample should be confirmed with MAT.
Literatur
1.
Zurück zum Zitat Faine S, et al., editors. Leptospira and leptospirosis. 2nd edn. Melbourne: MedSci; 1999. Faine S, et al., editors. Leptospira and leptospirosis. 2nd edn. Melbourne: MedSci; 1999.
2.
Zurück zum Zitat Bedernjak J, editor. Leptospiroze pri nas in v svetu. Murska Sobota: Pomurska založba; 1993. Bedernjak J, editor. Leptospiroze pri nas in v svetu. Murska Sobota: Pomurska založba; 1993.
3.
Zurück zum Zitat Palmer MF. Laboratory diagnosis of leptospirosis. Med Lab Sci. 1988;45:174–8. PubMed Palmer MF. Laboratory diagnosis of leptospirosis. Med Lab Sci. 1988;45:174–8. PubMed
4.
Zurück zum Zitat Avsic-Zupanc T, et al. Isolation of a strain of a Hantaan virus from a fatal case of hemorrhagic fever with renal syndrome in Slovenia. Am J Trop Med Hyg. 1994;51:393–400. PubMed Avsic-Zupanc T, et al. Isolation of a strain of a Hantaan virus from a fatal case of hemorrhagic fever with renal syndrome in Slovenia. Am J Trop Med Hyg. 1994;51:393–400. PubMed
5.
Zurück zum Zitat Winslow WE, et al. Evaluation of a commercial enzyme-linked immunosorbent assay for detection of immunoglobulin M antibody in diagnosis of human leptospiral infection. J Clin Microbiol. 1997;35:1938–42. PubMedCentralPubMed Winslow WE, et al. Evaluation of a commercial enzyme-linked immunosorbent assay for detection of immunoglobulin M antibody in diagnosis of human leptospiral infection. J Clin Microbiol. 1997;35:1938–42. PubMedCentralPubMed
6.
Zurück zum Zitat Levett PN, Whittington CU. Evaluation of the indirect hemagglutination assay for diagnosis of acute leptospirosis. J Clin Microbiol. 1998;36:11–4. PubMedCentralPubMed Levett PN, Whittington CU. Evaluation of the indirect hemagglutination assay for diagnosis of acute leptospirosis. J Clin Microbiol. 1998;36:11–4. PubMedCentralPubMed
7.
Zurück zum Zitat Goris MG, Boer KR, Duarte TA, Kliffen SJ, Haartskerl RA. Human leptospirosis trends, the Netherlands, 1925–2008. Emerg Infect Dis. 2013;19:371–8. PubMedCentralCrossRefPubMed Goris MG, Boer KR, Duarte TA, Kliffen SJ, Haartskerl RA. Human leptospirosis trends, the Netherlands, 1925–2008. Emerg Infect Dis. 2013;19:371–8. PubMedCentralCrossRefPubMed
8.
Zurück zum Zitat Cole JR, Sulzer CR, Pursell AR. Improved microtechnique for the leptospiral microscopic agglutination test. Appl Microbiol. 1973;25:976–80. PubMedCentralPubMed Cole JR, Sulzer CR, Pursell AR. Improved microtechnique for the leptospiral microscopic agglutination test. Appl Microbiol. 1973;25:976–80. PubMedCentralPubMed
9.
Zurück zum Zitat Murry PR, Baron JE, Jorgensen JH, editors. Manual of clinical microbiology. 9th edn. Washington: ASM Press; 2007. pp. 963–70.Levett PN: Leptospira. Murry PR, Baron JE, Jorgensen JH, editors. Manual of clinical microbiology. 9th edn. Washington: ASM Press; 2007. pp. 963–70.Levett PN: Leptospira.
10.
Zurück zum Zitat Ooteman MC, Vago AR, Koury MC. Evaluation of MAT, IgM ELISA and PCR methods for the diagnosis of human leptospirosis. J Microbiol Methods. 2006;65:247–57. CrossRefPubMed Ooteman MC, Vago AR, Koury MC. Evaluation of MAT, IgM ELISA and PCR methods for the diagnosis of human leptospirosis. J Microbiol Methods. 2006;65:247–57. CrossRefPubMed
11.
Zurück zum Zitat World Health Organisation. Human leptospirosis: guidance for diagnosis, surveillance and control. Geneva: International Leptospirosis Society; 2003. World Health Organisation. Human leptospirosis: guidance for diagnosis, surveillance and control. Geneva: International Leptospirosis Society; 2003.
14.
Zurück zum Zitat Blacksell SD, et al. Limited diagnostic capacities of two commercial assays for the detection of Leptospira Immunoglobulin M antibodies in Laos. Clin Vaccine Immunol. 2006;13:1166–9. PubMedCentralCrossRefPubMed Blacksell SD, et al. Limited diagnostic capacities of two commercial assays for the detection of Leptospira Immunoglobulin M antibodies in Laos. Clin Vaccine Immunol. 2006;13:1166–9. PubMedCentralCrossRefPubMed
15.
16.
Zurück zum Zitat Levett PN, Branch SL. Evaluation of two enzyme-linked immunosorbent assay methods for detection of immunoglobulin M antibodies in acute leptospirosis. Am J Trop Med Hyg. 2002;66:745–8. PubMed Levett PN, Branch SL. Evaluation of two enzyme-linked immunosorbent assay methods for detection of immunoglobulin M antibodies in acute leptospirosis. Am J Trop Med Hyg. 2002;66:745–8. PubMed
17.
Zurück zum Zitat Zochowski WJ, Palmer MF, Coleman TJ. An evaluation of three commercial kits for use as screening methods for the detection of leptospiral antibodies in the UK. J Clin Pathol. 2001;54:25–30. PubMedCentralCrossRefPubMed Zochowski WJ, Palmer MF, Coleman TJ. An evaluation of three commercial kits for use as screening methods for the detection of leptospiral antibodies in the UK. J Clin Pathol. 2001;54:25–30. PubMedCentralCrossRefPubMed
18.
Zurück zum Zitat Smits HL. International multicenter evaluation of the clinical utility of a dipstick assay for detection of Leptospira—specific immunoglobulin M antibodies in human serum specimens. J Clin Microbiol. 1999;37:2904–9. PubMedCentralPubMed Smits HL. International multicenter evaluation of the clinical utility of a dipstick assay for detection of Leptospira—specific immunoglobulin M antibodies in human serum specimens. J Clin Microbiol. 1999;37:2904–9. PubMedCentralPubMed
Metadaten
Titel
Evaluation of the immunochromatographic (Leptocheck) test for detection of specific antibodies against leptospires
verfasst von
univ. dipl. mikr. Daša Podgoršek
Tjaša Cerar
Mateja Logar
Gorazd Lešničar
Tatjana Remec
Zvonko Baklan
Emil Pal
Eva Ružić-Sabljić
Publikationsdatum
01.12.2015
Verlag
Springer Vienna
Erschienen in
Wiener klinische Wochenschrift / Ausgabe 23-24/2015
Print ISSN: 0043-5325
Elektronische ISSN: 1613-7671
DOI
https://doi.org/10.1007/s00508-015-0853-0