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Erschienen in: Wiener klinische Wochenschrift 17-18/2017

Open Access 01.09.2017 | case report

Combined metformin-associated lactic acidosis and euglycemic ketoacidosis

verfasst von: Verena Schwetz, Florian Eisner, Gernot Schilcher, Kathrin Eller, Johannes Plank, Alice Lind, Thomas R. Pieber, Julia K. Mader, Philipp Eller

Erschienen in: Wiener klinische Wochenschrift | Ausgabe 17-18/2017

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Summary

Background

In renal failure metformin can lead to lactic acidosis. Additional inhibition of hepatic gluconeogenesis by accumulation of the drug may aggravate fasting-induced ketoacidosis. We report the occurrence of metformin-associated lactic acidosis (MALA) with concurrent euglycemic ketoacidosis (MALKA) in three patients with renal failure.

Case presentations

Patient 1: a 78-year-old woman (pH = 6.89, lactic acid 22 mmol/l, serum ketoacids 7.4 mmol/l and blood glucose 63 mg/dl) on metformin and insulin treatment. Patient 2: a 79-year-old woman on metformin treatment (pH = 6.80, lactic acid 14.7 mmol/l, serum ketoacids 6.4 mmol/l and blood glucose 76 mg/dl). Patient 3: a 71-year-old man on metformin, canagliflozin and liraglutide treatment (pH = 7.21, lactic acid 5.9 mmol/l, serum ketoacids 16 mmol/l and blood glucose 150 mg/dl). In all patients, ketoacidosis receded on glucose infusion and renal replacement therapy.

Conclusion

This case series highlights the parallel occurrence of MALA and euglycemic ketoacidosis, the latter exceeding ketosis due to starvation, suggesting a metformin-triggered inhibition of gluconeogenesis. Affected patients benefit from glucose infusion counteracting suppressed hepatic gluconeogenesis.
Hinweise
Author contributions
V. Schwetz, J.K. Mader, P. Eller collected data, interpreted data and drafted the manuscript. F. Eisner, G. Schilcher, K. Eller, J. Plank, A. Lind, T.R. Pieber interpreted data, contributed to discussions, and critically revised the manuscript. All authors approved the final version of the manuscript. P. Eller is the guarantor of this work.
Abkürzungen
MALA
Metformin-associated lactic acidosis with concurrent euglycemic ketoacidosis
MALKA
Metformin-associated lactic acidosis with euglycemic ketoacidosis
CKD
Chronic kidney disease
ED
Emergency department
HR
Heart rate
SGLT2 inhibitor
Sodium-dependent glucose transporter 2 inhibitor

Background

Metformin is the most commonly used oral antihyperglycemic drug in type 2 diabetes. Metformin-associated lactic acidosis (MALA) is a rare but severe adverse effect in patients with renal failure. Besides elevation of lactate, acidosis has been attributed to uremia. In severe cases hemodialysis is recommended. Metformin increases the activity of AMP-dependent protein kinase, stimulating fatty acid oxidation, glucose uptake, non-oxidative metabolism, and reducing lipogenesis and gluconeogenesis [1], resulting in diminished hepatic glucose production and lower blood glucose levels. In an experimental setting, administration of metformin increased ketoacid production [2]. In renal failure and MALA, accumulation of metformin may lead to increased ketogenesis adding to the acid disturbance.
In this article we report three cases of metformin-associated lactic acidosis and euglycemic ketoacidosis (MALKA), diabetes and acute renal failure identified in 2015 at the intensive care unit (ICU) of a tertiary center. Serum beta-hydroxybutyrate levels (0.0–8.0 mmol/l) were measured by Precision Xtra Blood β‑Ketone Test Strips (Abbott Diabetes Care, Witney, UK).

Case series

Patient 1: a 78-year-old woman with a body mass index (BMI) of 26.3 kg/m2 on metformin and insulin therapy with chronic kidney disease (CKD) stage IIIa due to traumatic unilateral nephrectomy was admitted to the emergency department (ED) with nausea, vomiting, acute on chronic renal failure (creatinine 796.5 µmol/l, blood urea nitrogen 28.4 mmol/l) and metabolic acidosis with Kussmaul breathing (pH = 6.89, HCO3 = 5 mmol/l, pCO2 = 17.3 mm Hg or 2.3 kPa). The premedication included metformin 850 mg b.i. d. and biphasic insulin aspart b.i. d. (16–0–12 IU). On admission to the ICU, the patient was somnolent, the blood pressure (BP) was 103/45 mm Hg, body temperature 34.1 °C, oxygen saturation 97%, heart rate (HR) 76/min, arterial pO2 132 mm Hg (=17.6 kPa), base excess −30 mmol/l, anion gap 47 mmol/l and serum lactic acid level 22 mmol/l. Moreover, the patient displayed elevated serum ketoacids of 7.4 mmol/l and blood glucose (BG) levels of 63 mg/dl. Laboratory findings were as follows: C‑peptide 0.8 ng/ml (0.78–1.89), tyrosine phosphatase antibodies 0.0 U/ml (0.0–20.0), glutamic acid decarboxylase antibodies 0.3 U/l (0.0–9.5). Cranial computed tomography scan and chest X‑ray showed no pathologies. Initial treatment included hemodialysis, bicarbonate substitution, and glucose infusion. The high anion gap acidosis slowly receded during the following days (Fig. 1a). After initial stabilization, the patient acquired pneumonia requiring invasive mechanical ventilation and ongoing renal replacement therapy but finally passed away.
Patient 2: a 79-year-old woman (BMI 28.4 kg/m2) on metformin therapy with previous CKD IIIb on metformin 1000 mg b.i. d., candesartan, gabapentin, phenprocoumon, and pantoprazol was admitted to our ED. She had gastroenteritis with acute on chronic renal failure (creatinine: 796.5 µmol/l, blood urea nitrogen: 27.6 mmol/l), overt hyperkalemia of 7.7 mmol/l and metabolic acidosis with Kussmaul breathing (pH = 6.80, HCO3 = 5 mmol/l, pCO2 = 23 mm Hg or 3.1 kPa). On admission to the ICU, she had a BP of 165/71 mm Hg, a HR of 85/min, an oxygen saturation of 98%, a body temperature of 36.8 °C, an arterial pO2 of 175 mm Hg (23.3 kPa), a base excess of −27 mmol/l, and an anion gap of 25 mmol/l. The initial lactic acid level was 16 mmol/l. Moreover, the patient displayed elevated serum ketoacids of 6.4 mmol/l and a BG of 76 mg/dl. Diabetes-related laboratory findings were indicative of type 2 diabetes: C‑peptide 2.34 ng/ml (0.78–1.89), insulin antibodies 1.0 U/ml (0.0–9.0), tyrosine phosphatase antibodies 0.0 U/ml (0.0–20.0), glutamic acid decarboxylase antibodies 2.2 U/l (0.0–9.5). Chest X‑ray on admission was normal. Hemodialysis and parenteral glucose infusion was started and metabolic acidosis thus receded over time (Fig. 1b). Renal function slowly recovered, the patient was switched to an insulin regimen and discharged after 14 days.
Patient 3: a 71-year-old man (BMI 30.0 kg/m2) on metformin, canagliflozin, liraglutide, enalapril, allopurinol, pantoprazole and torasemid presented to the ED because of acute gastroenteritis lasting for 6 days. The laboratory analyses revealed acute renal failure (creatinine 1203 µmol/l, blood urea nitrogen 36.9 mmol/l) and metabolic acidosis with hyperventilation (pH = 7.16, HCO3 = 10 mmol/l, pCO2 = 20 mm Hg or 2.7 kPa). On admission, the BP was 111/38 mm Hg, HR 73/min, oxygen saturation 98%, body temperature 35.8 °C, arterial pO2 88 mm Hg (11.7 kPa), base excess −20 mmol/l, and the anion gap was 21.6 mmol/l. The initial lactic acid level was 5.9 mmol/l, serum ketoacids were ≥8 mmol/l at a blood glucose level of 150 mg/dl. Abdominal sonography and chest X‑ray revealed no pathologies. Continuous venovenous hemofiltration was started and parenteral glucose infusion initiated. Metabolic acidosis receded (Fig. 1c) and renal function slowly recovered. The patient was discharged after 9 days.

Conclusion

To conclude, we report on three critically ill patients with MALKA with metformin accumulation due to acute renal failure. The concurrent occurrence of lactic acidosis and ketoacidosis was reflected in an augmented anion gap. The underlying mechanism contributing to ketoacidosis-aggravated metabolic acidosis could be starvation; however, ketoacid production for alternative energy during starvation usually induces mild metabolic acidosis [3]. Beta-hydroxybutyrate levels in starvation [3] undercut the levels measured in our patients. In the cases presented, metformin-induced inhibition of gluconeogenesis and stimulation of fatty acid oxidation is a likely co-factor for ketogenesis in the absence of other known ketogenic situations, such as pregnancy [4, 5] or alcohol intake [5].
To the best of our knowledge, in the studies published on MALA, ketoacids have not been systematically measured and the anion gap was explained solely by lactate and uremia. In support of our postulated hypothesis of suppressed hepatic gluconeogenesis in MALA leading to euglycemic ketoacidosis, a study in rats has shown the induction of ketoacid production by metformin treatment [2]. Likewise, post-mortem biochemistry analysis of beta-hydroxybutyrate and glucose of the previously mentioned fatal case with metformin overdosing [6] supports our findings.
The mechanism of metformin-associated ketogenesis seems to differ from lack of insulin in type 1 diabetes. Parenteral glucose terminates starvation ketoacidosis and counteracts the suppressed hepatic gluconeogenesis in MALKA. Accordingly, patients with MALKA seem to benefit primarily from parenteral glucose infusion, reflected in a case report of starvation metabolic acidosis [7] and the management of euglycemic ketoacidosis in pregnancy [4]. Interestingly, new evidence suggests that SGLT2-inhibitors might also cause euglycemic ketoacidosis [8]. As the third patient was on canagliflozin and metformin treatment, discrimination of the cause of euglycemic ketoacidosis, i.e. SGLT2 inhibitor and/or biguanide therapy, is currently impossible in that patient.
Taken together, our case series highlights the parallel occurrence of MALA and euglycemic ketoacidosis, the latter exceeding ketosis observed during starvation, suggesting a metformin-triggered inhibition of gluconeogenesis. The occurrence of MALA should prompt investigations for euglycemic ketoacidosis, and euglycemic ketoacidosis should lead to measurement of serum lactate levels in patients on metformin. Furthermore, prospective studies need to clarify the importance of early implementation of glucose infusions in cases of MALKA.

Funding

This research did not receive any funding from the public or commercial sector.

Compliance with ethical guidelines

Conflict of interest

V. Schwetz, F. Eisner, G. Schilcher, K. Eller, J. Plank, A. Lind, T.R. Pieber, J.K. Mader, and P. Eller declare that they have no competing interests.

Ethical standards

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. Informed consent was obtained from all individual participants included in the study.
Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://​creativecommons.​org/​licenses/​by/​4.​0/​), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Literatur
1.
Zurück zum Zitat Shaw RJ, Lamia KA, Vasquez D, et al. The kinase LKB1 mediates glucose homeostasis in liver and therapeutic effects of metformin. Science. 2005;310:1642–6.CrossRefPubMedPubMedCentral Shaw RJ, Lamia KA, Vasquez D, et al. The kinase LKB1 mediates glucose homeostasis in liver and therapeutic effects of metformin. Science. 2005;310:1642–6.CrossRefPubMedPubMedCentral
2.
Zurück zum Zitat Tessari P, Tiengo A. Metformin treatment of rats with diet-induced overweight and hypertriglyceridemia decreases plasma triglyceride concentrations, while decreasing triglyceride and increasing ketone body output by the isolated perfused liver. Acta Diabetol. 2008;45:143–5.CrossRefPubMed Tessari P, Tiengo A. Metformin treatment of rats with diet-induced overweight and hypertriglyceridemia decreases plasma triglyceride concentrations, while decreasing triglyceride and increasing ketone body output by the isolated perfused liver. Acta Diabetol. 2008;45:143–5.CrossRefPubMed
3.
Zurück zum Zitat Owen OE, Smalley KJ, D’Alessio DA, Mozzoli MA, Dawson EK. Protein, fat, and carbohydrate requirements during starvation: anaplerosis and cataplerosis. Am J Clin Nutr. 1998;68:12–34.PubMed Owen OE, Smalley KJ, D’Alessio DA, Mozzoli MA, Dawson EK. Protein, fat, and carbohydrate requirements during starvation: anaplerosis and cataplerosis. Am J Clin Nutr. 1998;68:12–34.PubMed
4.
Zurück zum Zitat Frise CJ, Mackillop L, Joash K, Williamson C. Starvation ketoacidosis in pregnancy. Eur J Obstet Gynecol Reprod Biol. 2013;167:1–7.CrossRefPubMed Frise CJ, Mackillop L, Joash K, Williamson C. Starvation ketoacidosis in pregnancy. Eur J Obstet Gynecol Reprod Biol. 2013;167:1–7.CrossRefPubMed
6.
Zurück zum Zitat Bonsignore A, Pozzi F, Fraternali Orcioni G, Ventura F, Palmiere C. Fatal metformin overdose: case report and postmortem biochemistry contribution. Int J Legal Med. 2014;128:483–92.CrossRefPubMed Bonsignore A, Pozzi F, Fraternali Orcioni G, Ventura F, Palmiere C. Fatal metformin overdose: case report and postmortem biochemistry contribution. Int J Legal Med. 2014;128:483–92.CrossRefPubMed
7.
Zurück zum Zitat Toth HL, Greenbaum LA. Severe acidosis caused by starvation and stress. Am J Kidney Dis. 2003;42:E16–E9.CrossRefPubMed Toth HL, Greenbaum LA. Severe acidosis caused by starvation and stress. Am J Kidney Dis. 2003;42:E16–E9.CrossRefPubMed
8.
Zurück zum Zitat Peters AL, Buschur EO, Buse JB, Cohan P, Diner JC, Hirsch IB. Euglycemic diabetic ketoacidosis: a potential complication of treatment with sodium-glucose cotransporter 2 inhibition. Diabetes Care. 2015;38:1687–93.CrossRefPubMedPubMedCentral Peters AL, Buschur EO, Buse JB, Cohan P, Diner JC, Hirsch IB. Euglycemic diabetic ketoacidosis: a potential complication of treatment with sodium-glucose cotransporter 2 inhibition. Diabetes Care. 2015;38:1687–93.CrossRefPubMedPubMedCentral
Metadaten
Titel
Combined metformin-associated lactic acidosis and euglycemic ketoacidosis
verfasst von
Verena Schwetz
Florian Eisner
Gernot Schilcher
Kathrin Eller
Johannes Plank
Alice Lind
Thomas R. Pieber
Julia K. Mader
Philipp Eller
Publikationsdatum
01.09.2017
Verlag
Springer Vienna
Erschienen in
Wiener klinische Wochenschrift / Ausgabe 17-18/2017
Print ISSN: 0043-5325
Elektronische ISSN: 1613-7671
DOI
https://doi.org/10.1007/s00508-017-1251-6

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