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Erschienen in: Wiener klinische Wochenschrift 1-2/2017

01.01.2017 | original article

Pretransplantation monitoring of HLA antibodies by complement dependent cytotoxicity and Luminex-based assays

verfasst von: Nataša Katalinić, Marina Fućak, Tajana Crnić, Milena Ćurković, Alma Starčević, Sanja Balen

Erschienen in: Wiener klinische Wochenschrift | Ausgabe 1-2/2017

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Summary

Background

The introduction of more sensitive techniques, such as Luminex® for HLA antibody screening of patients awaiting organ transplantation has resulted in a better understanding of transplantation immunology and improvements in clinical practice.

Objective

The interpretation of the results obtained only by Luminex® can lead to inaccurate evaluation of a patient’s antibody status and unjustified rejection of a potential organ donor. The aim of this study was to demonstrate the benefits of performing HLA antibody screening in the sera of patients on the waiting list for organ transplantation by two different assays, complement dependent cytotoxicity (CDC) and Luminex®.

Methods

A retrospective analysis was performed on 563 pretransplant serum samples from 141 patients on the kidney transplantation waiting list in Rijeka, tested from March 2012 until March 2015. All samples were tested in parallel by the CDC assay and the Luminex®-based assay.

Results

Out of the 563 samples tested 302 (53.7%) tested negative for HLA antibodies and 88 (15.6%) positive by both assays. From 173 (30.7%) samples with discordant results 149 (26.5%) were CDC negative and Luminex® positive, while 24 (4.3%) were CDC positive and Luminex® negative. Among the Luminex positive patients seven did not experience any immunizing events.

Conclusion

Evaluation of the HLA antibody status in patients on a waiting list for organ transplantation should be based on the results of the both CDC and Luminex® (or other sensitive) assays in accordance to information about patient’s clinical status and exposure to immunizing events.
Literatur
1.
Zurück zum Zitat Lobashevsky AL. Methodological aspects of anti-human leukocyte antigen antibody analysis in solid organ transplantation. World J Transplant. 2014;4(3):153–67.CrossRefPubMedPubMedCentral Lobashevsky AL. Methodological aspects of anti-human leukocyte antigen antibody analysis in solid organ transplantation. World J Transplant. 2014;4(3):153–67.CrossRefPubMedPubMedCentral
3.
Zurück zum Zitat Terasaki PI, Cai J. Humoral theory of transplantation: further evidence. Curr Opin Immunol. 2005;17:541–5.CrossRefPubMed Terasaki PI, Cai J. Humoral theory of transplantation: further evidence. Curr Opin Immunol. 2005;17:541–5.CrossRefPubMed
4.
Zurück zum Zitat Vlad G, Ho EK, Vasilescu ER, et al. Relevance of different antibody detection methods for the prediction of antibody-mediated rejection and deceased-donor kidney allograft survival. Hum Immunol. 2009;70(8):589–94.CrossRefPubMed Vlad G, Ho EK, Vasilescu ER, et al. Relevance of different antibody detection methods for the prediction of antibody-mediated rejection and deceased-donor kidney allograft survival. Hum Immunol. 2009;70(8):589–94.CrossRefPubMed
5.
Zurück zum Zitat Meier-Kriesche HU, Kaplan B. Waiting time on dialysis as the strongest modifiable risk factor for renal transplant outcomes: a paired donor kidney analysis. Transplantation. 2002;74(10):1377–81.CrossRefPubMed Meier-Kriesche HU, Kaplan B. Waiting time on dialysis as the strongest modifiable risk factor for renal transplant outcomes: a paired donor kidney analysis. Transplantation. 2002;74(10):1377–81.CrossRefPubMed
6.
Zurück zum Zitat Süsal C, Opelz G. Impact of HLA matching and HLA antibodies in organ transplantation. A collaborative transplant study view. Methods Mol Biol. 2012;88:267–77.CrossRef Süsal C, Opelz G. Impact of HLA matching and HLA antibodies in organ transplantation. A collaborative transplant study view. Methods Mol Biol. 2012;88:267–77.CrossRef
7.
Zurück zum Zitat Jawdeh ABG, Cuffy MC, Alloway RR, Shields AR, Woodle ES. Desensitization in kidney transplantation: review and future perspectives. Clin Transplant. 2014;28(4):494–507.CrossRefPubMed Jawdeh ABG, Cuffy MC, Alloway RR, Shields AR, Woodle ES. Desensitization in kidney transplantation: review and future perspectives. Clin Transplant. 2014;28(4):494–507.CrossRefPubMed
8.
Zurück zum Zitat Paramesh AS, Zhang R, Baber J, et al. The effect of HLA mismatch on highly sensitized renal allograft recepients. Clin Transplant. 2010;24(6):E247–E252.CrossRefPubMed Paramesh AS, Zhang R, Baber J, et al. The effect of HLA mismatch on highly sensitized renal allograft recepients. Clin Transplant. 2010;24(6):E247–E252.CrossRefPubMed
9.
Zurück zum Zitat Terasaki PI, McClelland JD. Microdroplet assay of human serum cytotoxins. Nature. 1964;204:998–1000.CrossRefPubMed Terasaki PI, McClelland JD. Microdroplet assay of human serum cytotoxins. Nature. 1964;204:998–1000.CrossRefPubMed
10.
Zurück zum Zitat Gebel HM, Bray RA, Nicherson P. Pre-transplant assessment of donor-reactive, HLA-specific antibodies in renal transplantation: contraindication vs. risk. Am J Transplant. 2003;3:1488–500.CrossRefPubMed Gebel HM, Bray RA, Nicherson P. Pre-transplant assessment of donor-reactive, HLA-specific antibodies in renal transplantation: contraindication vs. risk. Am J Transplant. 2003;3:1488–500.CrossRefPubMed
11.
Zurück zum Zitat Patel R, Terasaki PI. Significance of the positive cross-match test in kidney transplantation. N Engl J Med. 1969;280:735–9.CrossRefPubMed Patel R, Terasaki PI. Significance of the positive cross-match test in kidney transplantation. N Engl J Med. 1969;280:735–9.CrossRefPubMed
12.
Zurück zum Zitat Morales-Buenrostro LE, Terasaki PI, Marino-Vázquez LA, et al. “Natural” human leukocyte antigen antibodies found in nonalloimmunized healthy males. Transplantation. 2008;86(8):1111–5.CrossRefPubMed Morales-Buenrostro LE, Terasaki PI, Marino-Vázquez LA, et al. “Natural” human leukocyte antigen antibodies found in nonalloimmunized healthy males. Transplantation. 2008;86(8):1111–5.CrossRefPubMed
13.
Zurück zum Zitat Süsal C, Ovens J, Mahmoud K, et al. No association of kidney graft loss with human leucocyte antigen antibodies detected exclusively by sensitive luminex-single antigen testing: a collaborative transplant study report. Transplantation. 2011;91(8):883–7.CrossRefPubMed Süsal C, Ovens J, Mahmoud K, et al. No association of kidney graft loss with human leucocyte antigen antibodies detected exclusively by sensitive luminex-single antigen testing: a collaborative transplant study report. Transplantation. 2011;91(8):883–7.CrossRefPubMed
14.
Zurück zum Zitat Grubić Z, Žunec R, Čečuk-Jelić E, Kerhin-Brkljačić V, et al. Polymorphism of HLA-A,-B,-DRB1,-DQA1 and -DQB1 haplotypes in Croatian population. Eur J Immunogenet. 2000;27(1):47–51.CrossRefPubMed Grubić Z, Žunec R, Čečuk-Jelić E, Kerhin-Brkljačić V, et al. Polymorphism of HLA-A,-B,-DRB1,-DQA1 and -DQB1 haplotypes in Croatian population. Eur J Immunogenet. 2000;27(1):47–51.CrossRefPubMed
15.
Zurück zum Zitat Mehra KN. The role of HLA matching and recipient sensitization in organ allograft outcom. In: Tait BD, editor. The HLA complex in biology and medicine: A resource book, 1st edn. Chapter 29. New Delhi: Jaypee Brothers Medical Publisher; 2010. pp. 491–509. Mehra KN. The role of HLA matching and recipient sensitization in organ allograft outcom. In: Tait BD, editor. The HLA complex in biology and medicine: A resource book, 1st edn. Chapter 29. New Delhi: Jaypee Brothers Medical Publisher; 2010. pp. 491–509.
16.
Zurück zum Zitat Gombos P, Opelz G, Scherer S, Morath C, Zeier M, Schemmer P, Süsal C. Influence of test technique on sensitization status of patients on the kidney transplant waiting list. Am J Transplant. 2013;13(8):2075–82.CrossRefPubMed Gombos P, Opelz G, Scherer S, Morath C, Zeier M, Schemmer P, Süsal C. Influence of test technique on sensitization status of patients on the kidney transplant waiting list. Am J Transplant. 2013;13(8):2075–82.CrossRefPubMed
17.
Zurück zum Zitat Sumitran-Holgersson S. HLA-specific alloantibodies and renal graft outcome. Nephrol Dial Transplant. 2001;16:897–904.CrossRefPubMed Sumitran-Holgersson S. HLA-specific alloantibodies and renal graft outcome. Nephrol Dial Transplant. 2001;16:897–904.CrossRefPubMed
18.
Zurück zum Zitat Süsal C, Dohler B, Sadeghi M, Ovens J, Opelz G. HLA antibodies and occurrence of early adverse events inthe modern era of transplantation: a collaborative transplant study report. Transplantation. 2009;87:1367–71.CrossRefPubMed Süsal C, Dohler B, Sadeghi M, Ovens J, Opelz G. HLA antibodies and occurrence of early adverse events inthe modern era of transplantation: a collaborative transplant study report. Transplantation. 2009;87:1367–71.CrossRefPubMed
19.
Zurück zum Zitat Everly MJ. Summarizing the use of donor specific anti-HLA antibody monitoring in transplant patients. Clin Transpl. 2011;(2011):333–6. Everly MJ. Summarizing the use of donor specific anti-HLA antibody monitoring in transplant patients. Clin Transpl. 2011;(2011):333–6.
20.
Zurück zum Zitat Tait BD, Süsal C, Gebel HM, Nickerson PW, et al. Consesus guidelines on the testing and clinical management issues associated with HLA and non-HLA antibodies in transplantation. Transplantation. 2013;95:19–47.CrossRefPubMed Tait BD, Süsal C, Gebel HM, Nickerson PW, et al. Consesus guidelines on the testing and clinical management issues associated with HLA and non-HLA antibodies in transplantation. Transplantation. 2013;95:19–47.CrossRefPubMed
21.
Zurück zum Zitat Pei R, Lee JH, Shih NJ, Chen M, Terasaki PI. Single human leucocyte antigen flow cytometry beads for accurate identification of human leucocyte antigen antibody specificities. Transplantation. 2003;75:43–9.CrossRefPubMed Pei R, Lee JH, Shih NJ, Chen M, Terasaki PI. Single human leucocyte antigen flow cytometry beads for accurate identification of human leucocyte antigen antibody specificities. Transplantation. 2003;75:43–9.CrossRefPubMed
22.
Zurück zum Zitat Colombo MB, Haworth SE, Poli F, et al. Luminex technology for anti-HLA antibody screening: evaluation on performance and of impact on laboratory routine. Cytometry B Clin Cytom. 2007;72((6):465–71.CrossRefPubMed Colombo MB, Haworth SE, Poli F, et al. Luminex technology for anti-HLA antibody screening: evaluation on performance and of impact on laboratory routine. Cytometry B Clin Cytom. 2007;72((6):465–71.CrossRefPubMed
23.
Zurück zum Zitat Tait BD, Hudson F, Cantwell L, Brewin G, et al. Review article: luminex technology for HLA antibody detection in organ transplantation. Nephrology (Carlton). 2009;14:247–54.CrossRef Tait BD, Hudson F, Cantwell L, Brewin G, et al. Review article: luminex technology for HLA antibody detection in organ transplantation. Nephrology (Carlton). 2009;14:247–54.CrossRef
24.
Zurück zum Zitat Pereira S, Perkins S, Lee JH, et al. Donor-specific antibody against denatured HLA-A1: non clinically significant? Human Immunol. 2011;72:492–8.CrossRef Pereira S, Perkins S, Lee JH, et al. Donor-specific antibody against denatured HLA-A1: non clinically significant? Human Immunol. 2011;72:492–8.CrossRef
25.
Zurück zum Zitat Tait BD, Hudson F, Brewin G, Cantwell L, et al. Solid phase HLA antibody detection technology – Challenges in interpretation. Tissue Antigens. 2010;76:87–95.PubMed Tait BD, Hudson F, Brewin G, Cantwell L, et al. Solid phase HLA antibody detection technology – Challenges in interpretation. Tissue Antigens. 2010;76:87–95.PubMed
Metadaten
Titel
Pretransplantation monitoring of HLA antibodies by complement dependent cytotoxicity and Luminex-based assays
verfasst von
Nataša Katalinić
Marina Fućak
Tajana Crnić
Milena Ćurković
Alma Starčević
Sanja Balen
Publikationsdatum
01.01.2017
Verlag
Springer Vienna
Erschienen in
Wiener klinische Wochenschrift / Ausgabe 1-2/2017
Print ISSN: 0043-5325
Elektronische ISSN: 1613-7671
DOI
https://doi.org/10.1007/s00508-016-1094-6

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