The effect of ozone treatment on experimental colon anastomosis in rats
Background: Colonic anastomosis is a common surgical procedure. Complications after such surgery are still a major problem despite advances in techniques. We aimed to evaluate the effects of pre-operative and postoperative ozone administration on the recovery of colonic anastomoses in rats in this study.
Methods: An end-to-end colon anastomosis was created in the left colon under anesthesia in all experiment group animals. The control group was left alone for 4 days. The other two groups were administered intraperitoneal ozone at a dose of 1 mg/kg once a day. Ozone therapy was started 24 h after surgery to one group (ozone) and 1 h before surgery to the other group (pre-ozone). Samples for biochemical measurements were obtained from the operation site after adhesion scoring was performed and the burst pressure measured.
Results: The burst pressure and OH-proline measurements in the ozone group were found to be significantly higher than in the other groups (p ˂ 0.05). These values were not different between the pre-ozone and control groups (p ˃ 0.05). The MDA values of the ozone group were significantly lower but the antioxidant enzyme levels (except superoxide dismutase) were significantly higher than the other two groups. The TNF-α and IL-6 levels of the ozone group were significantly lower while the IL-10 levels were significantly higher compared with the other two groups.
Conclusion: We therefore feel that ozone treatment after surgery could be important in terms of accelerating colon anastomosis recovery. Ozone administration supports recovery by reducing oxidative stress and proinflammatory cytokines.