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European Surgery

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01.12.2013 | original article

Impact of pneumoperitoneum on collagen I expression in vitro

verfasst von: T. F. Ulmer, MD, Dr. M. Binnebösel, A. Mossdorf, Prof. Dr. U. P. Neumann, Dr. R. Rosch

Erschienen in: European Surgery | Ausgabe 6/2013

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Summary

Background

There is little and contradictory data about the impact of laparoscopy on wound healing. In this experimental study, gas-related effects of pneumoperitoneum on collagen I mRNA synthesis were analysed separately in fibroblast cultures in vitro.

Methods

3T3 mouse fibroblasts were incubated for 1 h with either CO₂ or helium (gas flow: 1 l/min, pressure: 12 mmHg). Control fibroblasts were exposed to room air for 1 h. Hereafter, expression of collagen type IA1 was analysed by real-time PCR.

Results

Expression of collagen type IA1 analysed was 0.7 times lower after incubation with CO₂ when compared with controls. After helium insufflation, collagen type IA1 expression showed a 1.1-fold increase.

Conclusions

The observed gas-related influence on collagen synthesis might give evidence of a potential effect of laparoscopy on intra-abdominal wound healing.

Vorheriger Artikel The effect of harmonic scalpel, electrocautery, and scalpel use on early wound complications after modified radical mastectomy

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Titel: Impact of pneumoperitoneum on collagen I expression in vitro
verfasst von: T. F. Ulmer, MD
Dr. M. Binnebösel
A. Mossdorf
Prof. Dr. U. P. Neumann
Dr. R. Rosch
Publikationsdatum: 01.12.2013
Verlag: Springer Vienna
Erschienen in: European Surgery / Ausgabe 6/2013
Print ISSN: 1682-8631
Elektronische ISSN: 1682-4016
DOI: https://doi.org/10.1007/s10353-013-0232-x

Springer Medizin Österreich

Summary

Background

Methods

Results

Conclusions

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